Extremely halophilic archaeal isolates obtained from brine and sediment samples of solar salterns of Goa and Tamil Nadu, India were screened for accumulation of polyhydroxyalkanoates (PHA). Seven polymer accumulating haloarchaeal strains (TN4, TN5, TN6, TN7, TN9, TN10 and BBK2) were selected based on their growth and intensity of fluorescence when grown on 20 % NaCl synthetic medium supplemented with 2 % glucose and incorporated with Nile red dye. The polymer was quantified by conversion of PHA to crotonic acid which gave a characteristic absorption maxima at 235 nm. On the basis of phenotypic and genotypic characterization the cultures TN4, TN5, TN6, TN7, TN10 and BBK2 were grouped under genus Haloferax whereas isolate TN9 was grouped under the genus Halogeometricum. Growth kinetics and polymer accumulation studies revealed that the culture Halogeometricum borinquense strain TN9 accumulates PHA maximally at the mid-log phase, i.e. 5th day of growth (approx. 14 wt% PHA of CDW). Analysis of the polymer by IR, (1)H NMR and (13)C NMR confirmed it to be a homopolymer of 3-hydroxybutyrate.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s00792-013-0561-5 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A genome-scale metabolic model for the denitrifying bacterium Thauera sp. MZ1T accurately predicts degradation of pollutants and production of polymers.
PLoS Comput Biol
January 2025
Department of Pediatrics, University of California, San Diego, 9500 Gilman Drive, La Jolla, California, United States of America.
The denitrifying bacterium Thauera sp. MZ1T, a common member of microbial communities in wastewater treatment facilities, can produce different compounds from a range of carbon (C) and nitrogen (N) sources under aerobic and anaerobic conditions. In these different conditions, Thauera modifies its metabolism to produce different compounds that influence the microbial community.
View Article and Find Full Text PDFDevelopment of Antimicrobial Blends of Bacteria Nanocellulose Derived from Plastic Waste and Polyhydroxybutyrate Enhanced with Essential Oils.
Polymers (Basel)
December 2024
PRISM, Research Institute, Technological University of the Shannon, Midlands Midwest, Athlone, Co., Dublin Rd, N37 HD68 Westmeath, Ireland.
The escalating global concern regarding plastic waste accumulation and its detrimental environmental impact has driven the exploration of sustainable alternatives to conventional petroleum-based plastics. This study investigates the development of antimicrobial blends of bacterial nanocellulose (BNC) derived from plastic waste and polyhydroxyalkanoates (PHB), further enhanced with essential oils. The antimicrobial activity of the resulting BNC/PHB blends was tested in vitro against , , and .
View Article and Find Full Text PDFAssessing the efficiency and potential for internally reusing nitrogen-containing effluent in the PHA accumulation stage under low C/N conditions in a mixed-culture process.
Bioresour Technol
December 2024
School of Water Conservancy and Transportation, Zhengzhou University, Zhengzhou 450001, China. Electronic address:
Article Synopsis
- Polyhydroxyalkanoates (PHAs) are biodegradable plastics that can be produced through a mixed culture-based process, but ammonia nitrogen can hinder this production.
- This study explores ways to efficiently reuse ammonia nitrogen to enhance PHA synthesis and reduce waste.
- Results showed a significant increase in PHA production when using specific substrate and process conditions, while also effectively recycling ammonia without negatively affecting the mixed culture's properties.
Lignocellulosic biomass as promising substrate for polyhydroxyalkanoate production: Advances and perspectives.
Biotechnol Adv
December 2024
State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, PR China. Electronic address:
The depletion of fossil resources, coupled with global warming and adverse environmental impact of traditional petroleum-based plastics, have necessitated the discovery of renewable resources and innovative biodegradable materials. Lignocellulosic biomass (LB) emerges as a highly promising, sustainable and eco-friendly approach for accumulating polyhydroxyalkanoate (PHA), as it completely bypasses the problem of "competition for food". This sustainable and economically efficient feedstock has the potential to lower PHA production costs and facilitate its competitive commercialization, and support the principles of circular bioeconomy.
View Article and Find Full Text PDFProteomic analysis of natural photoheterotrophic mixed consortium for biohydrogen production under nongrowing conditions.
Bioresour Technol
December 2024
Bioprocesses Department, Instituto Politécnico Nacional, P.O. Box 07340, Mexico City, Mexico. Electronic address:
A biohydrogen and polyhydroxyalkanoates (PHA)-producing natural photoheterotrophic mixed culture composed mainly by Rhodopseudomonas palustris and Clostridium sp was studied by a proteomic analysis under non-growth conditions (nitrogen-absence and organic acids). Proteins in C. pasteurianum were upregulated, particularly those related to stress response.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!