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A novel method for detecting genetic biomarkers in blood-based liquid biopsies using surface plasmon resonance imaging and magnetic beads shows promise in cancer diagnosis and monitoring.
Talanta
January 2025
Department of Chemical Sciences, University of Catania, Viale Andrea Doria 6, 95122, Catania, Italy; INBB, Istituto Nazionale di Biostrutture e Biosistemi, Viale delle Medaglie d'Oro, 305, 00136, Roma, Italy. Electronic address:
Directly detecting biomarkers in liquid biopsy for diagnosis and personalized treatment plays a crucial role in managing cancer relapse and increasing survival rates. Typically, the standard analysis of circulating tumour DNA requires lengthy isolation, extraction, and amplification steps, leading to sample contamination, longer turnaround time and higher assay costs. Surface plasmon resonance is an emerging and promising technology for rapid and real-time dynamic biomarker monitoring in liquid biopsy.
View Article and Find Full Text PDFDevelopment and assessment of a novel magnetic nanoparticle antibody-conjugate and aptamer-based assay (MNp-Ab-Ap assay) for the rapid diagnosis of pleural tuberculosis.
Nanotheranostics
January 2025
Translational Research Laboratory, Department of Experimental Medicine and Biotechnology, Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh, India.
Article Synopsis
- Pleural tuberculosis (pTB) is hard to diagnose due to vague symptoms and limited testing options, prompting the development of a new assay using magnetic nanoparticles and antibodies to detect specific antigens related to the disease.
- The MNp-Ab-Ap assay was created by attaching antibodies to magnetic nanoparticles, enabling the capture of pTB antigens from pleural fluid, which were then identified using specialized aptamers.
- In testing, the MPT51-based variant of the assay showed a sensitivity of 66.6% and a high specificity of 95.4%, proving to be more effective than existing methods like the Xpert MTB/RIF test, suggesting it could significantly improve pTB diagnosis.
Co-crystal structure of Helicobacter pylori biotin protein ligase with biotinyl-5-ATP.
Acta Crystallogr F Struct Biol Commun
January 2025
Dartmouth Cancer Center, One Medical Center Drive, Lebanon, NH 03756, USA.
Article Synopsis
- Helicobacter pylori is a type 1 carcinogen linked to gastric ulcers and cancer, and research by the Seattle Structural Genomics Center for Infectious Disease focuses on potential treatments targeting this bacterium.
- The study reports on the purification and crystallization of H. pylori biotin protein ligase (HpBPL), an enzyme that plays a crucial role in important metabolic processes and helps H. pylori thrive in the acidic environment of the stomach.
- Despite having low sequence identity with similar proteins, HpBPL shares significant structural similarities with Mycobacterium tuberculosis biotin protein ligase, indicating potential for developing inhibitors that could be effective against HpBPL.
Electrochemistry-glucosemeter-smartphone integrated multi-mode biosensor for accurate detection of aflatoxin B1.
Anal Chim Acta
January 2025
College of Food Science and Technology, Henan Key Laboratory of Cereal and Oil Food Safety Inspection and Control, Henan University of Technology, Zhengzhou, 450001, China. Electronic address:
Background: Aflatoxin B1 (AFB1) is a widely distributed toxic contaminant in food and poses a serious threat to public health. Therefore, an accurate, simple, cost-effective and on-site assay method is needed for sensitive detection of AFB1. Aptamer shows great potential in the construction of biosensor due to its high specificity and affinity.
View Article and Find Full Text PDFQuantification of subcellular RNA localization through direct detection of RNA oxidation.
bioRxiv
November 2024
Department of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, USA.
Across cell types and organisms, thousands of RNAs display asymmetric subcellular distributions. The study of this process often requires quantifying abundances of specific RNAs at precise subcellular locations. To analyze subcellular transcriptomes, multiple proximity-based techniques have been developed in which RNAs near a localized bait protein are specifically labeled, facilitating their biotinylation and purification.
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