We conducted a laboratory study to evaluate the efficacy of control agents against small larvae, large larvae, and pupae of Aedes aegypti to determine an appropriate larvicide regime to employ in emergency dengue control programs. The control agents included Bacillus thuringiensis var. israelensis (Bti), pyriproxyfen (an insect growth regulator), a larvicidal oil, Aquatain AMF (polydimethylsiloxane, a monomolecular film), and temephos at the recommend application dosages and rates. Our results showed that Bti, pyriproxyfen, and temephos were efficacious (100% mortality) against larvae, irrespective of the instar stage, but not against pupae of Ae. aegypti (1.5-7.8% mortality). Aquatain AMF, on the other hand, was very effective at controlling the pupal stage (100% mortality), but had limited efficacy against small larvae (38.0% mortality) and large larvae (78.0% mortality). The larvicidal oil was effective against all immature stages (93.3-100% mortality). Therefore, we concluded that for effectively interrupting the dengue transmission cycle, larvicides that kill the pupal stage (Aquatain AMF or larvicidal oil) should be included in an emergency dengue control program in addition to Bti, pyriproxyfen, or temephos.
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http://dx.doi.org/10.7883/yoken.66.341 | DOI Listing |
Environ Sci Pollut Res Int
June 2024
Departamento de Morfologia e Fisiologia Animal (DMFA), Universidade Federal Rural de Pernambuco. Av, Dom Manoel de Medeiros S/N, Dois Irmãos, Recife, PE, 52171-900, Brazil.
Pyriproxyfen (PPF), Bacillus thuringiensis israelensis (BTI), and malathion (MLT) are widely used worldwide to control the population of mosquitos that transmit arboviruses. The current work aimed to evaluate the toxicity of these single pesticides and their binary mixtures of PPF + BTI, PPF + MLT, and MLT + BTI on the embryo-larval stage of zebrafish (Danio rerio) as an animal model. Epiboly, mortality, apical endpoints, affected animals, heart rate, morphometric, thigmotaxis, touch sensitivity, and optomotor response tests were evaluated.
View Article and Find Full Text PDFJ Med Entomol
January 2024
United States Department of Agriculture, Agricultural Research Service, Center for Medical, Agricultural, and Veterinary Entomology, 1600 SW 23rd Drive, Gainesville, FL 32608, USA.
Control of mosquito vector populations is primarily intended to reduce the transmission of pathogens they transmit. Use of chemical controls, such as larvicides, can have unforeseen consequences on adult traits if not applied properly. The consequences of under application of larvicides are little studied, specifically the impacts on pathogen infection and transmission by the vectors that survive exposure to larvicides.
View Article and Find Full Text PDFTrop Med Health
April 2021
Department of Hygiene and Public Health, Kansai Medical University, Hirakata, Osaka, Japan.
Background: Aedes aegypti, which is widely distributed in the Lao People's Democratic Republic (PDR), is the primary vector of arboviral diseases. Chemical insecticides have been intensively used to eliminate mosquito-borne diseases, resulting in the development of insecticide resistance. However, little is known about the insecticide resistance of mosquito populations in Lao PDR and the mechanisms responsible for it, which have important implications for vector management programs.
View Article and Find Full Text PDFJ Med Entomol
May 2020
Department of Vector Control, Center for Research, Diagnostic and Reference, Institute of Tropical Medicine Pedro Kourí, Havana, Cuba.
The chemical control of the mosquito is a great challenge worldwide, since several populations of this species are already resistant to traditional insecticides, such as temephos. In Brazil, alternative larvicides, such as () and pyriproxyfen, have been used more recently. In this study we evaluated the persistence of pyriproxyfen (Sumilarv 0.
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