Unlabelled: Tracking transmission of Mycobacterium tuberculosis strains in a population of humans and animals is the basis of modern epidemiology of tuberculosis. Molecular methods are used for the analysis of small patient population, as well as a global studies, which monitor the variation and geographic distribution of mycobacteria. A special role is played by genotyping methods that allow to control the spread of drug-resistant-TB and Beijing-TB. Beijing strains, especially drug-resistant clones, are regarded as one of the most dangerous mycobacteria. They have a high pathogenicity, the ability to rapidly acquire drug resistance and unpredictable response to treatment. The aim of this study was molecular and epidemiological analysis of M. tuberculosis Beijing strains isolated from patients with pre-XDR-TB, detected in Poland, and investigate the probable route of transmission of infection between patients.
Material And Methods: Material for analysis was a collection of more than 10,000 strains of M. tuberculosis collected in the years 2001-2011 in the National TB Reference Laboratory at the National Tuberculosis and Lung Diseases Research Institute in Warsaw. Analysis of phenotypic resistance to primary and secondary drugs allowed the selection of 47 pre-XDR strains, and genotyping--18 pre-XDR-Beijing strains. Molecular analysis included 2 genotyping methods--spoligotyping and MIRU-VNTR analysis.
Results: Genotyping results allowed the identification of 5 epidemiological groups of 13 patients with pre-XDR-Beijing-TB, between which transmission could occur. Based on the available epidemiological and demographic data, it was found that in isolated groups, 5 patients could be in close or casual contact.
Conclusions: This analysis shows that in Poland, one of the causes of drug resistance and the spread of M. tuberculosis Beijing type, is the transmission of infection between patients. This observation requires conducting active molecular and epidemiological investigations among patients, as well as studies of people with their environment.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Machine learning-enabled virtual screening indicates the anti-tuberculosis activity of aldoxorubicin and quarfloxin with verification by molecular docking, molecular dynamics simulations, and biological evaluations.
Brief Bioinform
November 2024
Institute of Medical Information, Chinese Academy of Medical Sciences and Peking Union Medical College, Chaoyang District, Beijing 100020, China.
Drug resistance in Mycobacterium tuberculosis (Mtb) is a significant challenge in the control and treatment of tuberculosis, making efforts to combat the spread of this global health burden more difficult. To accelerate anti-tuberculosis drug discovery, repurposing clinically approved or investigational drugs for the treatment of tuberculosis by computational methods has become an attractive strategy. In this study, we developed a virtual screening workflow that combines multiple machine learning and deep learning models, and 11 576 compounds extracted from the DrugBank database were screened against Mtb.
View Article and Find Full Text PDFAccuracy of ESAT6-CFP10 skin test compared with tuberculin skin test in a healthy population: a randomized, blind, parallel controlled phase III clinical study.
BMC Infect Dis
December 2024
Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, Jiangsu Province, PR China.
Background: This study aimed to evaluate the diagnostic performance of ESAT6-CFP10 (EC) skin test in healthy population and determine the factors influencing the booster effect.
Methods: We conducted a randomized, double-blind, parallel controlled trial in healthy population. The experiment was divided into two stages.
Evaluation of susceptibility to pyrazinamide and streptomycin, isoniazid, rifampin and ethambutol of Mycobacterium caprae strains isolated from European bison (Bison bonasus caucasicus) in the Bieszczady Mountains (Southern Poland).
Pol J Vet Sci
September 2024
Department of Food Hygiene and Public Health Protection, Institute of Veterinary Medicine, Warsaw University of Life Sciences (SGGW), Nowoursynowska 159, 02-776 Warsaw, Poland.
The material for drug resistance testing was 28 strains of Mycobacterium caprae isolated from tissue collected post mortem from a free-living Bieszczady Mountain European bison (Bison bonasus caucasicus) herd. All drug susceptibility tests were carried out on an automated Bactec mycobacterial growth indicator tube (MGIT) 960 system, using Bactec MGIT 960 streptomycin, isoniazid, rifampin and ethambutol (S.I.
View Article and Find Full Text PDFCavitary Pulmonary Tuberculosis in an Infant.
Cureus
November 2024
Pediatrics, Unidade Local de Saúde São João, Porto, PRT.
Tuberculosis (TB) continues to pose a significant health challenge globally and in Portugal. Diagnostic challenges persist, especially in infants, where TB often presents with atypical symptoms. A previously healthy three-month-old male infant from Vila Nova de Famalicão, Portugal, was admitted with cough, rhinorrhea, respiratory distress, and high-grade fever.
View Article and Find Full Text PDFIn silico study of bioactive compounds derived from Indonesian marine invertebrates as a novel antituberculosis agent.
Turk J Med Sci
December 2024
Research Center for Vaccine and Drugs, Research Organization for Health, National Research and Innovation Agency (BRIN), Cibinong, Bogor, Indonesia.
Background/aim: Tuberculosis (TB) has become the world's deadliest disease. The lack of an effective therapeutic drug to treat it is one of the obstacle for doctors. Today, multidrug-resistant TB cases are increasing.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!