AI Article Synopsis
- The study utilized ultra-performance liquid chromatography and mass spectrometry to analyze metabolites of 20(S)-protopanaxadiol (PPD) in rats after they were given the substance orally, identifying 23 metabolites in various biological fluids.
- Sixteen of these metabolites were newly discovered, shedding light on the complex metabolic processes of PPD, which include reactions like demethylation and glucuronidation.
- The findings enhance the understanding of PPD's metabolic pathways and suggest that the structural modification of PPD could lead to new chemical derivatives valuable for pharmacological research.
Article Abstract
In this study, ultra-performance liquid chromatography (UPLC)/quadrupole-time-of-flight mass spectrometry (QTOF-MS) was applied to the rapid analysis of 20(S)-protopanaxadiol (PPD) metabolites in rats after oral administration, enabling the structural characterization of 23 metabolites in plasma, bile, urine, and feces. 16 of these, including M1-M5, M9, and M11-M15, have not been previously reported. The results also indicated that demethylation, dehydration, dehydrogenation, oxidation, deoxidation, and glucuronidation were the major metabolic reactions of PPD in vivo. This study provides important information about the metabolism of PPD which will be helpful for fully understanding its mechanism of action. Furthermore, structural modification of PPD in vivo may aid in obtaining new chemical derivatives for pharmacological screening.
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| http://dx.doi.org/10.1016/j.jchromb.2013.03.028 | DOI Listing |
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