Introduction: For centuries, Semen Strychni (the ripened seeds of Strychnos nux-vomica) has been used extensively as a herbal medicine in Asian countries. However, the chemical composition of the dihydroindole-type alkaloids contained in processed Semen Strychni is not fully understood.
Objective: To develop an improved strategy using mass defect filtering (MDF) in combination with MS(n) analysis and theoretical calculations for identification and structural characterisation of dihydroindole-type alkaloids in processed Semen Strychni extracts.
Methods: The experimental work was conducted using a high-performance liquid chromatography coupled with electrospray ionisation ion trap time-of-flight mass spectrometry (HPLC-ESI/IT-TOF/MS) system. Upon acquisition of full-scan MS data, the potential dihydroindole-type alkaloids were screened using a well-defined mass defect range of 50 mDa. With the assistance of MS(n) analysis, the diagnostic fragment ions (DFIs) were used as primary screening references for targeting the characteristic analogues. For better discrimination of the isomers, quantum chemical calculations were utilised to provide additional structural information.
Results: Twenty-four dihydroindole-type alkaloids, including four that were previously not described, were tentatively identified.
Conclusion: A new, rapid and sensitive method was developed for the discovery and characterisation of dihydroindole-type alkaloids in extracts of processed Semen Strychni. The successful application of this method indicates a potential for adaptation to other classes of natural product from other sources.
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http://dx.doi.org/10.1002/pca.2457 | DOI Listing |
J Sep Sci
November 2019
Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), China Pharmaceutical University, Nanjing, P. R. China.
The ripened seeds of Strychnos nux-vomica L. have been extensively used as herbal medicines in Asian countries. Dihydroindole-type alkaloids are not only the active constituents but also the toxicants in Strychnos.
View Article and Find Full Text PDFBioanalysis
January 2014
Key Laboratory of Drug Quality Control & Pharmacovigilance, China Pharmaceutical University, Ministry of Education, Nanjing 210009, China.
Background: Brucine is a widely prescribed glycine antagonist, but a complete understanding of its metabolic pathway is still lacking. The present work represents the first investigation of in vivo metabolism of brucine in rats using LC-ESI-ion trap-TOF-MS.
Results: A total of 12 Phase I and five Phase II metabolites were tentatively identified.
Phytochem Anal
August 2014
Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing, 210009, China.
Introduction: For centuries, Semen Strychni (the ripened seeds of Strychnos nux-vomica) has been used extensively as a herbal medicine in Asian countries. However, the chemical composition of the dihydroindole-type alkaloids contained in processed Semen Strychni is not fully understood.
Objective: To develop an improved strategy using mass defect filtering (MDF) in combination with MS(n) analysis and theoretical calculations for identification and structural characterisation of dihydroindole-type alkaloids in processed Semen Strychni extracts.
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