AI Article Synopsis
- The Arp2/3 complex is crucial for cell movement and transporting vesicles, functioning as an actin filament nucleator.
- It is utilized in biochemical tests and structural studies due to its importance in cell biology.
- The purification method described involves extracting the complex from bovine thymus tissue as it is a eukaryotic assembly made of seven polypeptides.
Article Abstract
The Arp2/3 complex is an actin filament nucleator involved in cell motility and vesicle trafficking. Owing to the role the complex plays in important and fundamental cell biological processes, the purified complex is used in biochemical assays, reconstituted motility assays, and structural biology. As this is a eukaryotic complex assembled from seven polypeptides, the complex is purified from eukaryotic sources. Described here is a detailed method for purification of the complex from a mammalian tissue, bovine thymus.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3994882 | PMC |
| http://dx.doi.org/10.1007/978-1-62703-538-5_14 | DOI Listing |
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