AI Article Synopsis
- The study explores changes in the metabolic profiles of pancreatic cancer in mice before and after radiotherapy using high-resolution magnetic resonance spectroscopy and principal components analysis.
- The researchers injected pancreatic cancer cells into nude mice, exposed them to different radiation doses, and compared the metabolic profiles of normal and cancerous tissues.
- Results showed specific metabolites were altered due to cancer and radiation treatment, indicating the potential of these methods for early diagnosis and monitoring treatment response in pancreatic cancer.
Article Abstract
Aim: To investigate the metabolic profiles of xenograft pancreatic cancer before and after radiotherapy by high-resolution magic angle spinning proton magnetic resonance spectroscopy (HRMAS (1)H NMR) combined with principal components analysis (PCA) and evaluate the radiotherapeutic effect.
Methods: The nude mouse xenograft model of human pancreatic cancer was established by injecting human pancreatic cancer cell SW1990 subcutaneously into the nude mice. When the tumors volume reached 800 mm(3), the mice received various radiation doses. Two weeks later, tumor tissue sections were prepared for running the NMR measurements. (1)H NMR and PCA were used to determine the changes in the metabolic profiles of tumor tissues after radiotherapy. Metabolic profiles of normal pancreas, pancreatic tumor tissues, and radiation- treated pancreatic tumor tissues were compared.
Results: Compared with (1)H NMR spectra of the normal nude mouse pancreas, the levels of choline, taurine, alanine, isoleucine, leucine, valine, lactate, and glutamic acid of the pancreatic cancer group were increased, whereas an opposite trend for phosphocholine, glycerophosphocholine, and betaine was observed. The ratio of phosphocholine to creatine, and glycerophosphocholine to creatine showed noticeable decrease in the pancreatic cancer group. After further evaluation of the tissue metabolic profile after treatment with three different radiation doses, no significant change in metabolites was observed in the (1)H NMR spectra, while the inhibition of tumor growth was in proportion to the radiation doses. However, PCA results showed that the levels of choline and betaine were decreased with the increased radiation dose, and conversely, the level of acetic acid was dramatically increased.
Conclusion: The combined methods were demonstrated to have the potential for allowing early diagnosis and assessment of pancreatic cancer response to radiotherapy.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3710423 | PMC |
| http://dx.doi.org/10.3748/wjg.v19.i26.4200 | DOI Listing |
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