Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.2323/jgam.59.257 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Construction of an expression platform for fungal secondary metabolite biosynthesis in Penicillium crustosum.
Appl Microbiol Biotechnol
July 2024
Institut Für Pharmazeutische Biologie Und Biotechnologie, Fachbereich Pharmazie, Philipps-Universität Marburg, Robert-Koch-Straße 4, 35037, Marburg, Germany.
Filamentous fungi are prolific producers of bioactive natural products and play a vital role in drug discovery. Yet, their potential cannot be fully exploited since many biosynthetic genes are silent or cryptic under laboratory culture conditions. Several strategies have been applied to activate these genes, with heterologous expression as one of the most promising approaches.
View Article and Find Full Text PDFIn Vivo Genome Editing in Type I and II Methanotrophs Using a CRISPR/Cas9 System.
ACS Synth Biol
February 2023
BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Biodiscovery Institute, University of Nottingham, University Park, Nottingham NG7 2RD, U.K.
Methanotrophic bacteria are Gram-negative, aerobic organisms that use methane as their sole source of carbon and energy. In this study, we constructed and exemplified a CRISPR/Cas9 genome editing system and used it to successfully make gene deletions and insertions in the type I methanotroph Bath and the type II methanotroph OBBP. High frequencies of gene deletions and insertions were achieved in combination with homology-directed repair.
View Article and Find Full Text PDFDesign and Construction of Portable CRISPR-Cpf1-Mediated Genome Editing in 168 Oriented Toward Multiple Utilities.
Front Bioeng Biotechnol
September 2020
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.
is an important Gram-positive bacterium for industrial biotechnology, which has been widely used to produce diverse high-value added chemicals and industrially and pharmaceutically relevant proteins. Robust and versatile toolkits for genome editing in are highly demanding to design higher version chassis. Although the () CRISPR-Cas9 has been extensively adapted for genome engineering of multiple bacteria, it has many defects, such as higher molecular weight which leads to higher carrier load, low deletion efficiency and complexity of sgRNA construction for multiplex genome editing.
View Article and Find Full Text PDFHospital-acquired infections with Pseudomonas aeruginosa have become a great challenge in caring for critically ill and immunocompromised patients. The cause of high mortality is the presence of multi-drug resistant (MDR) strains, which confers a pressing need for vaccines. Although vaccines against P.
View Article and Find Full Text PDFModeling regional sustainable development scenarios using the Urbanization and Eco-environment Coupler: Case study of Beijing-Tianjin-Hebei urban agglomeration, China.
Sci Total Environ
November 2019
Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:
China's rapid urbanization has produced problems of excessive resource use and environmental pollution, threatening the country's sustainable development. Previous studies mainly focused on empirical observation of the interactions between urbanization and the eco-environment, mainly using econometric models which lacked detailed explanations of the coupling mechanisms between various elements. No quantitative models have been developed to describe the complex nonlinear relationships between various elements, so our understanding of urbanization and eco-environment coupling is vague, and therefore not conducive to coordinating the relationship between them.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!