Purpose: Acellular nerve allograft is a new option for bridging nerve defects that allows appropriate diameter matching. The aim of the study was to compare the histologic and functional recovery of nerve defects treated with acellular nerve allograft versus cabled sural nerve autograft.
Method: Fifty-four Sprague-Dawley rats were divided into one of three experimental groups. A unilateral 10 mm sciatic nerve defect was created and repaired with an acellular nerve allograft (Group A), three cabled sural nerve autografts in antidromic orientation (Group B), and the newly created segmental defect in antidromic orientation (reversed autograft) (Group C). Two rats in each group we evaluated histologically at 6 weeks while the rest of the groups were tested histologically and functionally at 12 weeks.
Results: There were no differences in histomorphometry between the groups at 6 weeks, but at 12 weeks at mid-graft there were differences. Group C had the highest fiber count which was statistically greater when compared to Group A (P = 0.023) and when compared to Group B (P = 0.001). The average normalized maximum isometric tetanic force (ITF) was 52 ± 2.9% for Group A, 34.1 ± 4.2% for Group B, and 51.3 ± 3.3% for Group C at 12 weeks. There was no statistical difference between Groups A and C, but Group A was statistically greater when compared to B, and when Group C was compared to B.
Conclusion: In conclusion, acellular nerve allograft demonstrated equal functional recovery when compared to reversed autograft (control), and superior recovery compared to the cabled nerve autograft.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/micr.22102 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Decellularization of fish tissues for tissue engineering and regenerative medicine applications.
Regen Biomater
November 2024
Zhejiang Top-Medical Medical Dressing Co. Ltd, Wenzhou, Zhejiang 325025, China.
Decellularization is the process of obtaining acellular tissues with low immunogenic cellular components from animals or plants while maximizing the retention of the native extracellular matrix structure, mechanical integrity and bioactivity. The decellularized tissue obtained through the tissue decellularization technique retains the structure and bioactive components of its native tissue; it not only exhibits comparatively strong mechanical properties, low immunogenicity and good biocompatibility but also stimulates neovascularization at the implantation site and regulates the polarization process of recruited macrophages, thereby promoting the regeneration of damaged tissue. Consequently, many commercial products have been developed as promising therapeutic strategies for the treatment of different tissue defects and lesions, such as wounds, dura, bone and cartilage defects, nerve injuries, myocardial infarction, urethral strictures, corneal blindness and other orthopedic applications.
View Article and Find Full Text PDFA chitosan/acellular matrix-based neural graft carrying mesenchymal stem cells to promote peripheral nerve repair.
Stem Cell Res Ther
December 2024
Departments of Neurosurgery, The First Center of Chinese, PLA General Hospital, Beijing, China.
Background: Treatment of peripheral nerve defects is a major concern in regenerative medicine. This study therefore aimed to explore the efficacy of a neural graft constructed using adipose mesenchymal stem cells (ADSC), acellular microtissues (MTs), and chitosan in the treatment of peripheral nerve defects.
Methods: Stem cell therapy with acellular MTs provided a suitable microenvironment for axonal regeneration, and compensated for the lack of repair cells in the neural ducts of male 8-week-old Sprague Dawley rats.
Preliminary study on the preparation of lyophilized acellular nerve scaffold complexes from rabbit sciatic nerves with human umbilical cord mesenchymal stem cells.
World J Stem Cells
December 2024
Department of Orthopedics, Children's Hospital of Fudan University & National Children's Medical Center, Shanghai 201102, China.
Background: The gold standard of care for patients with severe peripheral nerve injury is autologous nerve grafting; however, autologous nerve grafts are usually limited for patients because of the limited number of autologous nerve sources and the loss of neurosensory sensation in the donor area, whereas allogeneic or xenografts are even more limited by immune rejection. Tissue-engineered peripheral nerve scaffolds, with the morphology and structure of natural nerves and complex biological signals, hold the most promise as ideal peripheral nerve "replacements".
Aim: To prepare allogenic peripheral nerve scaffolds using a low-toxicity decellularization method, and use human umbilical cord mesenchymal stem cells (hUC-MSCs) as seed cells to cultivate scaffold-cell complexes for the repair of injured peripheral nerves.
Challenges in the surgical treatment of neuroma in continuity in the upper extremity using human acellular nerve allografts.
J Plast Reconstr Aesthet Surg
November 2024
Department of Hand Surgery, Herlev/Gentofte University Hospital of Copenhagen, Hospitalsvej 1, 2900 Hellerup, Denmark; University of Copenhagen, Faculty of Health and Medical Sciences, Institute of Clinical Medicine, Blegdamsvej 3B, 2200 Copenhagen, Denmark.
The restoration of nerve function after the injury might be complicated by the development of a disorganized fibrous mass-a neuroma. This results in sensory and/or motor deficits and pain that can be severely debilitating. Surgical excision of the painful neuroma may leave a gap, which can be bridged using autografts or allografts.
View Article and Find Full Text PDFManagement of Aesthetical and Functional Complications after Total Parotidectomy. First Long-Term Experiences with Dermal Matrix Surgimend ® in Patient Affected by Malignant Parotid Tumors.
J Maxillofac Oral Surg
December 2024
Maxillofacial Surgery UnitDepartmentof Medicine and SurgeryDepartment of Neurosciences, Reproductive and Odontostomatological Sciences, University Federico II, Via Sergio Pansini 5, 80131 Naples, Italy.
Background: This is an observational cohort study on patients affected by malignant parotid tumors treated with total parotidectomy. The aim of our work is to analyze and compare the effects and complications after parotidectomy, using or not SurgiMend ®.
Methods: 40 patients were retrospectively enrolled between September 2014 and June 2020.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!