[Act of Arc siRNA via lentivirus on the memory consolidation of amygdala-kindled rats].

Objective: To explore the memory consolidation related role of gene Arc in amygdala-kindled rats.

Methods: A total of 60 male amygdala-kindled rats were divided equally into 3 groups: 0.9% sodium chloride injection, null vector lentivirus injection and silencing lentivirus injection. Quantitative polymerase chain reaction (qPCR) and Western blot were performed to confirm the silencing efficiency of Arc. And the passive avoidance test and Morris' water maze were used to test the memory consolidation ability of rats in three groups.

Results: The memory consolidation ability became significantly impaired in the Arc silencing group in both of passive avoidance test and water maze comparing to the other groups. And no significant difference existed in memory consolidation ability between 0.9% sodium chloride and null vector lentivirus injection groups.

Conclusion: The ability of memory consolidation is impaired in Arc silencing amygdala-kindled rats and Arc may play a significant role in the consolidation of memory in amygdala-kindled rats.