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Apoptotic M540 bodies present in human semen interfere with flow cytometry-assisted assessment of sperm DNA fragmentation and oxidation.
Basic Clin Androl
October 2021
Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
Background: The use of flow cytometry (FC) to evaluate sperm DNA fragmentation via deoxynucleotidyl transferase terminal fluorescein dUTP nick-end labeling (TUNEL) has shown inconsistencies compared with conventional fluorescent microscopic analyses. It has been hypothesized that the observed discrepancies could be attributed to the presence of apoptotic bodies that can be labeled with merocyanine 540, the so-called M540 bodies. In order to verify this hypothesis and determine the accuracy of our in-house FC-assisted evaluation of spermatozoa parameters, we used FC to evaluate both the fragmentation of sperm DNA using the TUNEL assay and the oxidation of sperm DNA using the 8-OHdG assay on semen samples with or without M540 bodies.
View Article and Find Full Text PDFRole of epididymal anti sticking factor in sperm capacitation.
Biochem Biophys Res Commun
August 2015
Division of Cryobiology, Centre for Rural and Cryogenic Technologies, Jadavpur University, Kolkata 700032, West Bengal, India. Electronic address:
Sperm capacitation depends on several features like hormones, ions, intracellular signaling, sperm associated molecules, etc. Anti sticking factor (ASF) is a novel sperm surface associated glycoprotein isolated from epididymal plasma. Function of ASF in vivo has not been revealed yet.
View Article and Find Full Text PDFIdentification of apoptotic bodies in equine semen.
Reprod Domest Anim
April 2014
Cell Physiology Research Group, Department of Physiology, University of Extremadura, Caceres, Spain.
Apoptosis in the testis is required to ensure an efficient spermatogenesis. However, sometimes, defective germ cells that are marked for elimination during this process escape elimination in the testes, giving rise to ejaculates with increased percentages of abnormal and apoptotic spermatozoa and a high percentage of apoptotic bodies. Apoptosis markers in the ejaculate have been associated with low fertility, either in animals or humans.
View Article and Find Full Text PDFThe apoptotic pathway in fertile and subfertile men: a case-control and prospective study to examine the impact of merocyanine 540 bodies on ejaculated spermatozoa.
Fertil Steril
April 2013
Instituto Nacional de Perinatologia Isidro Espinosa de los Reyes, Mexico City, Mexico.
Objective: To evaluate the presence of merocyanine 540 (M540) bodies and their impact on the measurement of apoptotic biomarkers in human spermatozoa.
Design: Case-control, prospective study.
Setting: Academic centers.
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