AI Article Synopsis
- The paper introduces a compact imaging instrument, only 30 mm in length, designed to record high-speed fluorescent signals from brain tissues with a sensitivity of 0.1% ΔF/F.
- This device allows researchers to study neuronal activity in awake and freely moving animals, eliminating the need for anesthesia and restraint.
- It features an integrated illumination system, a wide field fluorescence microscope, and a CMOS image sensor, capable of imaging at 900 frames per second, showcased in experiments on the somatosensory cortex of mice during whisker movements.
Article Abstract
In this paper we present a fully self-contained imaging instrument (30 mm overall length) that is capable of recording high speed and detect relatively small fluorescent signals (0.1% ΔF/F) from brain tissues potentially containing genetically-encoded sensors or dyes. This device potentially enables the study of neuronal activity in awake and mobile animals during natural behaviors without the stress and suppression of anesthesia and restraint. The device is a fully self-contained illumination system, wide field fluorescence microscope (~ 4.8 mm² FOV-25 um lateral resolution-1.8 × magnification-0.39 NA) and CMOS image sensor (32 × 32). The total weight of the system is 10 g and is capable of imaging up to 900 fps. We present voltage dye RH1692 experiments using the system to study the somatosensory cortex of mice during whisker movements using an air puff.
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| http://dx.doi.org/10.1109/TBCAS.2012.2226174 | DOI Listing |
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