Avian coronavirus (commonly known as Infectious bronchitis virus [IBV]) is of major economic importance to commercial chicken producers worldwide. Due to the existence of multiple serotypes and variants of the virus that do not cross-protect, it is important to diagnose circulating serotypes and choose the right vaccine type for successful protection. In an effort to improve conventional diagnostic tests, a microsphere-based assay was developed and evaluated for simultaneous detection of the most common IBV vaccine serotypes in the United States: Arkansas (Ark), Connecticut (Conn), Massachusetts (Mass), Delaware (DE072), and Georgia 98 (GA98). The analytical specificity and sensitivity, and diagnostic specificity and sensitivity, were evaluated. The microsphere-based assay was highly specific to designated serotypes and generated reproducible data. Comparing the microsphere-based assay to nucleotide sequencing, the 2 methods agreed more than 93% (kappa value > .77). In addition, the microsphere-based assay could detect coinfections in clinical samples. The results demonstrate the utility of the microsphere-based assay as a rapid and accurate diagnostic tool with the potential for high throughput diagnosis.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1177/1040638713493906 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Microspheres based on galactomannan and Spondias purpurea L. extract to increase antifungal and antibiofilm efficacy against Candida spp.
Int J Biol Macromol
January 2025
Laboratory of Polymers and Materials Innovation, Department of Organic and Inorganic Chemistry, Sciences Center, Federal University of Ceará, Campus of Pici, Zip Code 60440-900 Fortaleza, CE, Brazil. Electronic address:
The ongoing problem of an increasing resistance of Candida spp. to available antifungals, has made it necessary the search for new therapeutic alternatives. The aim of this work was to develop a microsphere based on Caesalpinia ferrea galactomannan and Spondias purpurea L.
View Article and Find Full Text PDFFluorescent microsphere-based strip for sensitive and quantitative detection of etomidate and metomidate.
Analyst
January 2025
International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.
In this research, we fabricated a sensitive monoclonal antibody (mAb) 2C3 that targeted etomidate (ET) and metomidate (MT) to establish a lateral-flow immunoassay (LFIA) that incorporated fluorescent microsphere sensors, enabling both the qualitative and quantitative detection of ET and MT within 10 min. Analysis indicated that the visual colorimetric values for ET and MT in water samples were 0.3 μg kg, respectively, with quantitative detection ranges of 0.
View Article and Find Full Text PDFMultiplex sample-sparing assay for detecting type-specific antibodies to Zika and dengue viruses: an assay development and validation study.
Lancet Microbe
December 2024
Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill, NC, USA. Electronic address:
Background: Serology for dengue viruses (DENV) and Zika virus (ZIKV) has been hindered by antibody cross-reactivity, which limits the utility of these tests for surveillance and assessment of sero-status. Our aim was to develop a multiplexed IgG-based assay with increased accuracy to assess the history of previous DENV and ZIKV infections.
Methods: We developed and assessed the analytical performance of a sample-sparing, multiplexed, microsphere-based serological assay using domain III of the envelope protein (EDIII) of DENV serotypes 1-4 and ZIKV, the most variable region between each virus.
Development of a latex microsphere-based lateral flow immunoassay for the diagnosis of schistosomiasis japonica.
PLoS Negl Trop Dis
December 2024
Molecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
Article Synopsis
- Zoonotic schistosomiasis, specifically caused by Schistosoma japonicum, is a significant health issue in countries like China, the Philippines, and Indonesia, highlighting the need for effective diagnostic tools to aid control strategies.
- The study evaluated five recombinant antigens for diagnostic use, ultimately developing a latex microsphere lateral flow immunoassay (LFIA) with promising results: a sensitivity of 80.6% and specificity of 98.0% in diagnosing schistosomiasis japonica.
- The LFIA demonstrated superior prevalence detection compared to traditional methods, revealing a higher prevalence of schistosomiasis in the tested population, indicating its potential as a rapid point-of-care diagnostic tool.
Discovery of multiple bee-hazardous pesticides in ornamental plants via the Bee-Plex multi-target microsphere screening method.
J Hazard Mater
November 2024
Wageningen Food Safety Research, Akkermaalsbos 2, 6708 WB, Wageningen, The Netherlands. Electronic address:
Exposure to pesticides is one of the main drivers of global bee decline. However, the occurrence of pesticides in bee-attracting crops remains underexposed due to the lack of efficient on-site screening approaches for multi-analyte monitoring. Utilizing color-encoded superparamagnetic microspheres, we constructed a portable 8-plex indirect competitive microsphere-based immunoassay for the simultaneous determination of multiple bee-hazardous residues (Bee-Plex).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!