Background: Dolosigranulum pigrum is a commensal inhabitant of the upper respiratory tract suspected to be responsible for ocular infections but no well-described case of D. pigrum corneal infection has been reported. Herein culture and PCR-sequencing-based investigations of corneal scraping specimens confirmed D. pigrum keratitis in three patients.
Case Presentation: Three elderly patients presented with unilateral keratitis. None was a corneal-contact lens wearer, one had previous cataract surgery and another suffered rheumatoid arthritis sicca syndrome. Culturing the corneal scraping specimen was positive for two cases and PCR-sequencing of bacterial 16S rDNA in the presence of negative controls identified D. pigrum in three cases. The two D. pigrum isolates were in-vitro susceptible to penicillin G, amoxicillin, doxycycline, rifampicin and gentamicin. In all cases, surgical treatment of corneal thinning was necessary, but corneal perforation occurred in two cases despite intensive antimicrobial treatment with ticarcillin, gentamicin and vancomycin or levofloxacin eye drops leading to enucleation in one case.
Conclusions: D. pigrum is the likely cause of corneal infection in three patients, with effective antibiotic treatment in two patients.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3710215 | PMC |
| http://dx.doi.org/10.1186/1471-2415-13-31 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The Human Nasal Microbiome: A Perspective Study During the SARS-CoV-2 Pandemic in Malta.
Microorganisms
December 2024
The BioArte Ltd., Life Science Park, Triq San Giljan, 3000 San Gwann, Malta.
The human respiratory tract is colonized by a complex microbial community that helps maintain respiratory health and plays a crucial role in defending the host from infections. Respiratory viruses have been demonstrated to alter microbiota composition, resulting in opportunistic species expansion, and increasing the disease severity and host susceptibility to bacterial co-infections. This study aims to examine the compositional differences in the nasal microbiota between SARS-CoV-2-infected and non-infected patients.
View Article and Find Full Text PDFSignatures of lower respiratory tract microbiome in children with severe community-acquired pneumonia using shotgun metagenomic sequencing.
J Microbiol Immunol Infect
November 2024
Department of Pediatrics, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan. Electronic address:
Background: Severe community-acquired pneumonia was associated with high morbidity and mortality in children. However, species-level microbiome of lower airway was sparse, and we used shotgun metagenomic next-generation sequencing to explore microbial signatures.
Methods: We conducted a prospective cohort study to recruit children under 18 who required admission to an intensive care unit for community-acquired pneumonia between December 2019 and February 2022.
Septic shock and death due to Dolosigranulum pigrum bacteremia: a case report.
Jpn J Infect Dis
October 2024
Tone Chuo Hospital, Japan.
We report the case of a 77-year-old Japanese woman with neuromyelitis optica spectrum disorder, treated with 15 mg of prednisolone daily, who presented with acute septic shock and altered consciousness due to Dolosigranulum pigrum bacteremia. Intravenous extracellular fluids and broad-spectrum antibiotics were administered as an initial treatment. However, the patient died shortly after admission.
View Article and Find Full Text PDFThe Staphylococcus aureus-antagonizing human nasal commensal Staphylococcus lugdunensis depends on siderophore piracy.
Microbiome
October 2024
Cluster of Excellence EXC 2124 "Controlling Microbes to Fight Infections (CMFI)", University of Tübingen, Tübingen, Germany.
Background: Bacterial pathogens such as Staphylococcus aureus colonize body surfaces of part of the human population, which represents a critical risk factor for skin disorders and invasive infections. However, such pathogens do not belong to the human core microbiomes. Beneficial commensal bacteria can often prevent the invasion and persistence of such pathogens by using molecular strategies that are only superficially understood.
View Article and Find Full Text PDFNasal microbionts differentially colonize and elicit cytokines in human nasal epithelial organoids.
bioRxiv
September 2024
Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA.
Article Synopsis
- Nasal colonization by certain bacteria increases infection risk, while others are linked to health; this study used human nasal epithelial organoids (HNOs) to explore these dynamics.
- HNOs were successfully colonized with three bacterial species for up to 48 hours, showing minimal harm and allowing for the bacteria to localize in the mucus.
- The research identified specific immune responses to the bacterial species, indicating that some bacteria trigger inflammation while others modulate immune signaling without causing infection.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!