The complete mitogenome sequence of Spinibarbus sinensis was determined using long PCR reactions. The genome is 16,591 bp in length, including 13 typical vertebrate protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and a control region. Except for eight tRNA and ND6 genes, all other mitochondrial genes are encoded on the heavy strand. The gene order and composition of S. sinensis was similar to that of most other vertebrates. The descending order of the base composition on heavy strand was 32.0% A, 25.5% T, 26.6% C, 15.9% G, with a relatively lower level of G and a slight AT bias of 57.5%. The codon usage followed the typical vertebrate mitochondrial pattern (ATG or GTG for start codon and TAA or TAG for stop codon). There are 10 regions of gene overlap totaling 30 bp and 13 intergenic spacer regions totaling 69 bp.
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