AI Article Synopsis
- The study investigates the effects of psoralen (PSO) combined with ultraviolet A (UVA) on the apoptosis of human leukemia NB4 cells and the associated signaling pathways.
- The research involved treating NB4 cells with varying concentrations of PSO and UVA exposure times, with apoptosis rates measured using flow cytometry and cell structure analyzed via electron microscopy.
- Results indicate that the combination of PSO and UVA increases apoptosis in a dose- and time-dependent manner, particularly with 40 μg/mL PSO and 5 minutes of UVA, and enhances the expression of Caspase-3 and Caspase-8 proteins involved in the apoptosis process.
Article Abstract
Objective: To study the regulatory effects of psoralen (PSO) plus ultraviolet A (UVA), which is PUVA, on cell apoptosis of human leukemia cell line NB4 and signal pathway of cell apoptosis.
Methods: Human leukemia cell line NB4 was cultured in vitro. The NB4 cells were treated with PSO extracted from Chinese medicine psoralea fruits at different concentrations (0, 5, 10, 20 and 40 microL) plus UVA of wave length 360 nm at different irradiation time points (0 and 5 min). The apoptosis ratio was detected by flow cytometry (FCM). The ultrastructure changes were observed using transmission electron microscope (TEM). The expressions of Caspase-8 and Caspase-8 protein were detected by immunocytochemical method (ICC).
Results: After treatment of PSO at different concentrations with a 0 and 5-min exposure of UVA, the apoptosis rate of NB4 cells increased dose-and time-dependently, and was up to peak after treatment of PSO at 40 microg/mL with 5-min exposure of UVA. An interaction was shown between the two factors (P <0. 01). There were obvious morphological apoptosis of NB4 cells under TEM after treated with PUVA. The expressions of Caspase-3 and Caspase-8 protein were up-regulated by PSO, UVA, and PUVA, but the effects of PUVA on Caspase-3 protein were stronger than PSO and UVA at 12 h time-dependently (P <0.01).An interaction was shown between the concentration of PSO and time of UVA (P <0.01).
Conclusions: The optimal combination of PUVA was PSO in 40 microg/mL and 5-min exposure of UVA. PUVA could induce the apoptosis of NB4 cells and in vitro activate Caspase-3 and Caspase-8 genes.
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