AI Article Synopsis
- The study aimed to investigate the effects of probiotics on survival rates in septic rats induced by cecal ligation and puncture.
- Probiotics treatment significantly increased the survival of septic rats in a dose-dependent manner, with higher doses leading to better outcomes compared to the control group.
- The treatment also resulted in reduced bacterial counts in the blood and ascites, particularly for harmful bacteria like Escherichia coli and Staphylococcus aureus, and reduced inflammation in intestinal tissues.
Article Abstract
Aim: To investigate the benefits of probiotics treatment in septic rats.
Methods: The septic rats were induced by cecal ligation and puncture. The animals of control, septic model and probiotics treated groups were treated with vehicle and mixed probiotics, respectively. The mixture of probiotics included Bifidobacterium longum, Lactobacillus bulgaricus and Streptococcus thermophilus. We observed the survival of septic rats using different amounts of mixed probiotics. We also detected the bacterial population in ascites and blood of experimental sepsis using cultivation and real-time polymerase chain reaction. The severity of mucosal inflammation in colonic tissues was determined.
Results: Probiotics treatment improved survival of the rats significantly and this effect was dose dependent. The survival rate was 30% for vehicle-treated septic model group. However, 1 and 1/4 doses of probiotics treatment increased survival rate significantly compared with septic model group (80% and 55% vs 30%, P < 0.05). The total viable counts of bacteria in ascites decreased significantly in probiotics treated group compared with septic model group (5.20 ± 0.57 vs 9.81 ± 0.67, P < 0.05). The total positive rate of hemoculture decreased significantly in probiotics treated group compared with septic model group (33.3% vs 100.0%, P < 0.05). The population of Escherichia coli and Staphylococcus aureus in ascites of probiotics treated group were decreased significantly compared with that of septic model group (3.93 ± 0.73 vs 8.80 ± 0.83, P < 0.05; 2.80 ± 1.04 vs 5.39 ± 1.21, P < 0.05). With probiotics treatment, there was a decrease in the scores of inflammatory cell infiltration into the intestinal mucosa in septic animals (1.50 ± 0.25 vs 2.88 ± 0.14, P < 0.01).
Conclusion: Escherichia coli and Staphylococcus aureus may be primary pathogens in septic rats. Probiotics improve survival of septic rats by suppressing these conditioned pathogens.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3703194 | PMC |
| http://dx.doi.org/10.3748/wjg.v19.i25.4053 | DOI Listing |
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