AI Article Synopsis
- Quality assessment in flow cytometry differs from chemical analyte measurement and requires continuous monitoring of known patients for reliable results.
- A study analyzed blood samples from 32 HIV patients over years, calculating the stability of various cell subset percentages and absolute counts, resulting in low coefficient variations, indicating good reliability.
- Regular monitoring of compliant patients offers a cost-effective alternative to expensive repeatability studies for validating flow cytometry procedures.
Article Abstract
Background. Quality assessment in flow cytometry cannot obey the same rules as those applicable to the measurement of chemical analytes. However, regular follow-up of known patients may provide a robust in-house control of cell subsets evaluation. Methods. Sequential blood samples assessed for 32 HIV patients over several years and showing good stability were retrospectively assessed to establish coefficient of variations of the percentages of CD3+, CD4+, CD8+ cells and CD4+ absolute counts. Results. Mean relative standard variations for the whole cohort were of 0.04, 0.14, 0.08 and 0.18 for CD3%, CD4% CD8% and CD4 absolute counts respectively. Discussion. In-house follow up of regularly checked compliant patients is a good alternative to traditional and costly repeatability and reproducibility studies for the validation of routine flow cytometry. © 2013 Clinical Cytometry Society.
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| http://dx.doi.org/10.1002/cytob.21112 | DOI Listing |
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