Appl Microbiol Biotechnol
Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas-UNICAMP, Rua Monteiro Lobato 255, Cidade Universitária Zeferino Vaz, 13083-862 Campinas, São Paulo, Brazil.
Published: August 2013
Mycobacterium abscessus is an important hospital-acquired pathogen involved in infections associated with medical, surgical, and biopharmaceutical materials. In this work, we investigated the pressure-induced inactivation of two strains [2544 and American Type Culture Collection (ATCC) 19977] of M. abscessus in combination with different temperatures and pH conditions. For strain 2544, exposure to 250 MPa for 90 min did not significantly inactivate the bacteria at 20 °C, whereas at -15 °C, there was complete inactivation. Exposure to 250 MPa at ≥60 °C caused rapid inactivation, with no viable bacteria after 45 min. With 45 min of exposure, there were no viable bacteria at any temperature when a higher pressure (350 MPa) was used. Extremes of pH (4 or 9) also markedly enhanced the pressure-induced inactivation of bacteria at 250 MPa, with complete inactivation after 45 min. In comparison, exposure of this strain to the disinfecting agent glutaraldehyde (0.5 %) resulted in total inactivation within 5 min. Strain 19977 was more sensitive to high pressure but less sensitive to glutaraldehyde than strain 2544. These results indicate that high hydrostatic pressure in combination with other physical parameters may be useful in reducing the mycobacterial contamination of medical materials and pharmaceuticals that are sensitive to autoclaving.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1007/s00253-013-5067-7 | DOI Listing |
Sci Rep
January 2025
School of Resources and Civil Engineering, Northeastern University, Shenyang, 110819, China.
Based on the symmetric initiation mechanism of double-wing cracks in coal rock mass induced by high-pressure electro-recoil water pressure, fracturing experiments have been performed on coal rock mass under different water pressures and discharge conditions using high-voltage electric pulse hydraulic fracturing devices. Combined with CT scans, the crack spatial distribution inside the post-break coal rock mass was analyzed and found that the edge of the water injection hole is prone to produce double-wing cracks along the drilling hole diameter. ABAQUS is used to verify the physical test and extend the test conditions, the geometric parameter change, morphological expansion rule and crack initiation mechanism of double-wing crack in coal rock mass under different discharge conditions and ground stress conditions are studied.
View Article and Find Full Text PDFBMC Biol
January 2025
CAS Key Laboratory of Marine Ecology and Environmental Sciences, and Center of Deep Sea Research, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China.
Background: Lindaspio polybranchiata, a member of the Spionidae family, has been reported at the Lingshui Cold Seep, where it formed a dense population around this nascent methane vent. We sequenced and assembled the genome of L. polybranchiata and performed comparative genomic analyses to investigate the genetic basis of adaptation to the deep sea.
View Article and Find Full Text PDFbioRxiv
December 2024
Department of Biology, University of Miami, 1301 Memorial Drive, Coral Gables, FL 33146.
Animals alter their behavior in response to changes in the environment. Upon encountering hyperosmotic conditions, the nematode worm initiates avoidance and cessation of egg-laying behavior. While the sensory pathway for osmotic avoidance is well-understood, less is known about how egg laying is inhibited.
View Article and Find Full Text PDFCells under high confinement form highly polarized hydrostatic pressure-driven, stable leader blebs that enable efficient migration in low adhesion, environments. Here we investigated the basis of the polarized bleb morphology of metastatic melanoma cells migrating in non-adhesive confinement. Using high-resolution time-lapse imaging and specific molecular perturbations, we found that EGF signaling via PI3K stabilizes and maintains a polarized leader bleb.
View Article and Find Full Text PDFFoods
January 2025
Graduate School of Science and Technology, Niigata University, 8050 Ikarashi 2 nocho, Nishi-ku, Niigata 950-2181, Japan.
High-pressure treatment was utilized in this study to produce high-quality, reduced-sodium pork gels with desirable texture and sensory properties, addressing the challenge of maintaining quality in low-sodium meat products to meet health-conscious consumer demands. High-pressure treatment applied within the range of 150-200 MPa significantly reduced cooking loss while maintaining moisture content and provided an ideal network structure for reduced-sodium pork gels. High-pressure treatment at up to 100-200 MPa, in combination with added sodium chloride and sodium polyphosphate, was evaluated for its effects on gel texture, with results indicating that high-pressure treatment significantly improved breaking stress (increased by 10.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!
© LitMetric 2025. All rights reserved.