Purpose: To compare the relative performance, in an in vitro assay, of a standard dentifrice (Colgate Cavity Protection) with an experimental anhydrous 0.454% w/w stannous fluoride dentifrice at protecting the dentin smear layer against dietary acids.
Methods: Artificial smear layers were created on bovine dentin, contacted with slurries of the dentifrices, then exposed to a dietary acid (Coca Cola) for periods up to 10 minutes. Both the stability of the smear layer and tubule occlusion were assessed qualitatively by scanning electron microscopy and confocal laser scanning microscopy.
Results: Smear layers treated with the standard dentifrice were removed after 2 minutes of exposure to cola with the smear plugs progressively eroded by longer acid challenges, leaving patent tubules. Treatment with the experimental stannous fluoride dentifrice provided extensive protection of the smear layer against cola for up to 10 minutes, with very few tubules unoccluded.
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J Dent
March 2025
Clinical Trials Group, Bristol Dental School, University of Bristol, Lower Maudlin Street, Bristol, BS1 2LY, UK. Electronic address:
Objectives: This study was designed to evaluate the effectiveness of a multi-step oral hygiene intervention among removal partial denture (RPD) wearers.
Methods: A 12-week, single-blind, randomised, 2-armed, parallel-group, controlled trial was conducted with 49 RPD-wearing participants with mild-moderate gingivitis. The intervention group received stannous fluoride toothpaste, sodium fluoride mouth rinse, and an antibacterial denture cleanser foam.
Front Dent Med
November 2024
Global Oral Care R&D, The Procter & Gamble Company, Mason, OH, United States.
Objective: To determine whether outer membrane vesicles (OMVs) of the periodontal pathogen () can infect gingival keratinocytes and stimulate reactive oxygen species (ROS) production, and to assess whether stannous fluoride (SnF), stannous chloride (SnCl) or 0.454% SnF toothpaste diluents can inhibit OMV infection.
Methods: OMVs were isolated from culture and their morphology was characterized using scanning electron microscopy and transmission electron microscopy.
Microorganisms
December 2024
Oral Care Product Development, The Procter & Gamble Company, Cincinnati, OH 45202, USA.
Various ingredients are utilized to inhibit the growth of harmful bacteria associated with cavities, gum disease, and bad breath. However, the precise mechanisms by which these ingredients affect the oral microbiome have not been fully understood at the molecular level. To elucidate the molecular mechanisms, a high-throughput bacterial transcriptomics study was conducted, and the gene expression profiles of six common oral bacteria, including two Gram-positive bacteria (, ) and four Gram-negative bacteria (, , , and ), were analyzed.
View Article and Find Full Text PDFClin Oral Investig
December 2024
Department of Pedodontics, Faculty of Dentistry, Izmir Katip Celebi University, Izmir, Turkey.
Objectives: This study aims to comparatively assess the preventive and protective effects of the self-assembling peptide P-4 on enamel erosion and evaluate the potential for enamel surface recovery when professional products are combined with home-use dental-care products during the erosive process.
Materials And Methods: Ninety-nine bovine incisors were divided into nine groups: a control group, four groups with the application of professional-products [P-4 peptide (Curodont-Repair), stannous/Sn containing solution (8% Sn), casein-phosphopeptide-amorphous-calcium-phosphate fluoride/CPP-ACPF (MI Varnish), sodium fluoride/NaF (Profluorid)] and four groups with the combination of professional products and home-use daily dental care products [P-4 peptide (Curodont Repair + Curodont Protect), stannous ions containing agents (8% Sn+Emofluor Gel Intensive-Care), CPP-ACPF (MI Varnish + MI Paste Plus), NaF (Profluorid + ReminPro)]. Professional products were applied once before a five-day erosive cycle, involving six 2-minute citric-acid exposures per day.
Biosens Bioelectron
March 2025
School of Precision Instrument and Opto-electronics Engineering, Tianjin University, Tianjin, 300072, China.
Biomarker detection has emerged as an essential complementary approach for early-stage screening of tumors. Conventional methods are constrained by bulky systems, cumbersome operation steps, and low detection accuracy. Here, we demonstrate a dual-resonance optimally configured lossy mode resonance (LMR) immunoprobe for detecting prostate-specific antigen (PSA), a biomarker for prostate cancer (PCa).
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