We developed a highly sensitive chemiluminescent (CL) assay for hydrogen peroxide using 10-methyl-9-(phenoxycarbonyl) acridinium fluorosulfonate (PMAC) that produced chemiluminescence under neutral conditions and applied it to an enzyme immunoassay (EIA). One picomole of hydrogen peroxide could be detected using the optimized PMAC-CL method and 6.2 × 10(-20) mol β-D-galactosidase (β-gal) could be detected by combining an indoxyl derivative substrate and the proposed PMAC-CL method. This highly sensitive CL β-gal assay was applied to an EIA for thyroid-stimulating hormone (TSH) using β-gal as a label enzyme; 0.02-100.0 μU/mL TSH in human serum could be assayed directly and with high reproducibility.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/bio.2555 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Unipolar Barrier Photodetectors Based on Van Der Waals Heterostructure with Ultra-High Light On/Off Ratio and Fast Speed.
Adv Sci (Weinh)
January 2025
Information Materials and Intelligent Sensing Laboratory of Anhui Province, Key Laboratory of Structure and Functional Regulation of Hybrid Materials of Ministry of Education, Institutes of Physical Science and Information Technology, Anhui University, 111 Jiu Long Road, Hefei, 230601, China.
Unipolar barrier architecture is designed to enhance the photodetector's sensitivity by inducing highly asymmetrical barriers, a higher barrier for blocking majority carriers to depressing dark current, and a low minority carrier barrier without impeding the photocurrent flow through the channel. Depressed dark current without block photocurrent is highly desired for uncooled Long-wave infrared (LWIR) photodetection, which can enhance the sensitivity of the photodetector. Here, an excellent unipolar barrier photodetector based on multi-layer (ML) graphene (G) is developed, WSe, and PtSe (G-WSe-PtSe) van der Waals (vdW) heterostructure, in which extremely low dark current of 1.
View Article and Find Full Text PDFFeasibility study of hyperspectral colposcopy as a novel tool for detecting precancerous cervical lesions.
Sci Rep
January 2025
Research Institute for Applied Microelectronics (IUMA), University of Las Palmas de Gran Canaria (ULPGC), Las Palmas de Gran Canaria, Spain.
Cervical cancer remains a major global health concern, with a specially alarming incidence in younger women. Traditional detection techniques such as the Pap smear and colposcopy often lack sensitivity and specificity and are highly dependent on the experience of the gynaecologist. In response, this study proposes the use of Hyperspectral Imaging, a pioneering technology that combines traditional imaging with spectroscopy to provide detailed spatial and spectral information.
View Article and Find Full Text PDFIntegrated approach for estimating climate change impacts on CO sink capacity of inland waterbodies using hydrodynamic modelling and GIS analysis.
Sci Rep
January 2025
Department of Irrigation and Hydraulics Engineering, Faculty of Engineering, Tanta University, Tanta, Egypt.
As one of their key regulatory ecosystem functions, inland lakes serve as CO sinks. The CO sink capacity of inland lakes depends on their water temperature and salinity as well as their water volume which are all highly sensitive to climate conditions. This paper aims to quantitatively estimate the change in the CO sink capacity of Wadi El-Rayan Lakes under climate change scenarios.
View Article and Find Full Text PDFClinical manifestation and laboratory data of illicit drugs versus other substances intoxication in children.
Sci Rep
January 2025
Golestan Research Center of Gastroentrology and Hepatology & Stem Cell Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
Children are highly sensitive to toxins which can damage their organs and lead to death. Investigating the main causes of intoxication could reduce mortality and morbidity in children. In this cross-sectional study, the documents of all poisoned patients (214 cases) admitted to the emergency department of Taleghani children`s Hospital between April 2020 and 2023 were investigated.
View Article and Find Full Text PDFMesoporous carbon nanospheres-assisted amplified electrochemiluminescence for L-cysteine detection.
Anal Biochem
January 2025
Key Laboratory of Green and Precise Synthetic Chemistry and Applications, Ministry of Education, Anhui Provincial Key Laboratory of Synthetic Chemistry and Applications, College of Chemistry and Materials Science, Huaibei Normal University, Huaibei, Anhui 235000, PR China. Electronic address:
Luminol-loaded mesoporous carbon nanospheres (MCs@LU) were utilized to develop a highly sensitive electrochemiluminescence (ECL) sensor for the detection of L-cysteine (L-Cys). L-Cys acted as the coreactant of luminol, and the pore confinement effect of mesoporous carbons (MCs) resulted in a robust ECL signal. Upon optimization, a linear correlation between the ECL intensity and L-Cys concentration was observed over the range of 5.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!