AI Article Synopsis
- Type B GABA receptors (GABAB-Rs) are essential for synaptic transmission, and their surface expression may be influenced by the Nogo receptor 1 (NgR1).
- Silencing NgR1 led to a specific increase in GABAB R1 and R2 proteins without affecting GABAA receptor levels, and this increase was linked to the mTOR pathway.
- NgR1 knockout mice showed higher levels of GABAB R2 and GIRK1 proteins in the hippocampus, indicating that NgR1 plays a role in modulating synaptic transmission through G protein-coupled receptors and channels.
Article Abstract
Background: Type B GABA receptors (GABA Rs) play a critical role in synaptic transmission. We carried out studies to determine whether neuronal cell surface expression of GABAB-Rs might be regulated by the Nogo receptor 1 (NgR1).
Results: siRNA knock-down of NgR1 resulted in a selective increase of GABAB R1 and GABAB R2 protein without altering the expression of GABAA receptor or GAD65. The increase in GABAB receptor subunits was unaccompanied by a change in mRNA, but inhibition of mTOR by rapamycin blocked the increase in GABAB protein. NgR1 siRNA also caused an increase in G protein coupled inwardly rectifying potassium channel (GIRK1). The increase in GABAB receptor and GIRK1 channel proteins was in the plasma membrane, determined by cell surface biotinylation. In NgR1 knockout mice, the amount of GABAB R2 and GIRK1 in hippocampus-derived synaptosomes was increased.
Conclusions: Together these findings suggest that NgR1 mediated modulation of synaptic transmission may be accomplished, at least in part, through modulation of G protein coupled receptors and channels.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3734105 | PMC |
| http://dx.doi.org/10.1186/1756-6606-6-30 | DOI Listing |
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