Purified neuraminidase preparation from Erysipelothrix rhusiopathiae is studied biochemically. The main physicochemical characteristics of the enzyme are determined: molecular mass, isoelectric point, electrophoretic mobility, chemical nature, UV spectrum, substrate specificity, etc. The obtained data are compared to those for other bacterial neuraminidases.
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Chembiochem
December 2024
Department of Chemistry, University of Alberta, Edmonton, Alberta, T6G 2G2, Canada.
Glycoconjugates are a vast class of biomolecules implicated in biological processes important for human health and disease. The structural complexity of glycoconjugates remains a challenge to deciphering their precise biological roles and for their development as biomarkers and therapeutics. Human glycoconjugates on the outside of the cell are modified with sialic (neuraminic) acid residues at their termini.
View Article and Find Full Text PDFLangmuir
October 2024
The Institute of Chemistry and Center of Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
is a pathogenic bacterium that contains the surface-bound neuraminidase, . has two domains that interact with sialosides. It is hard to determine the contribution of each domain separately on catalysis or binding.
View Article and Find Full Text PDFJ Biol Chem
October 2024
Department of Biochemistry & Microbiology, University of Victoria, Victoria, British Columbia, Canada. Electronic address:
Sialic acids are commonly found on the terminal ends of biologically important carbohydrates, including intestinal mucin O-linked glycans. Pathogens such as Clostridium perfringens, the causative agent of necrotic enteritis in poultry and humans, have the ability to degrade host mucins and colonize the mucus layer, which involves removal of the terminal sialic acid by carbohydrate-active enzymes (CAZymes). Here, we present the structural and biochemical characterization of the GH33 catalytic domains of the three sialidases of C.
View Article and Find Full Text PDFProc Natl Acad Sci U S A
September 2024
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138.
Elevated bacterial sialidase activity in the female genital tract is strongly associated with poor health outcomes including preterm birth and bacterial vaginosis (BV). These negative effects may arise from sialidase-mediated degradation of the protective mucus layer in the cervicovaginal environment. Prior biochemical studies of vaginal bacterial sialidases have focused solely on the BV-associated organism .
View Article and Find Full Text PDFEur J Med Chem
October 2024
China International Science and Technology Cooperation Base of Food Nutrition/Safety and Medicinal Chemistry, College of Biotechnology, Tianjin University of Science & Technology, No. 29, 13th Avenue, TEDA, Tianjin, 300457, China. Electronic address:
Influenza remains a global public health threat, and the development of new antivirals is crucial to combat emerging drug-resistant influenza strains. In this study, we report the synthesis and evaluation of a sialyl lactosyl (TS)-bovine serum albumin (BSA) conjugate as a potential multivalent inhibitor of the influenza virus. The key trisaccharide component, TS, was efficiently prepared via a chemoenzymatic approach, followed by conjugation to dibenzocyclooctyne-modified BSA via a strain-promoted azide-alkyne cycloaddition reaction.
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