AI Article Synopsis
- Novozym 435, an enzyme from Candida antarctica, was utilized to produce ethyl esters in a solvent-free environment for the first time.
- The study focused on how the substrate-ethanol ratio and the stability of the biocatalyst affected the synthesis process.
- Advanced techniques like infrared spectroscopy, nuclear magnetic resonance, and mass spectrometry confirmed the structure of the resulting compounds, achieving a high reaction yield of 90% or more under optimal conditions.
Article Abstract
Novozym 435, lipase B from Candida antarctica, was used in this study for the production of ethyl esters. For the first time, trans-hydroxy-fatty acid ethyl esters were synthesized in vitro in solvent-free media. We studied the effects of the substrate-ethanol molar ratio and enzyme synthetic stability of the biocatalyst. To determine the structure of the formed compounds, Fourier transformed infrared spectroscopy, nuclear magnetic resonance, and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry were used, three less time-consuming structural techniques. trans-Hydroxy-fatty acid ethyl esters were synthesized with a reaction yield of 90 % or higher with optimal reaction conditions.
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| http://dx.doi.org/10.1007/s00253-013-5059-7 | DOI Listing |
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