Background: In routine histopathology, xylene has been used in tissue processing and staining. Presently health hazards of xylene are well documented and a safer substitute is an absolute necessity.
Aim: The present study was conducted to evaluate the efficacy of mineral oil as a deparaffinizing agent when compared to that of xylene by using hematoxylin and eosin (H&E) staining.
Materials And Methods: Thirty paraffin-embedded tissue blocks were randomly selected and two sections were taken from each block. Thirty sections were stained with conventional H&E method (group A) using xylene as deparaffinizing agent and 30 were stained with xylene free method using refined mineral oil (group B). Sections were blinded and analyzed by two pathologists using the parameters of uniformity, clarity and intensity of nuclear and cytoplasmic staining respectively (satisfactory = score 1, unsatisfactory = score 0). Score ≥ 4 was considered to be adequate for diagnosis.
Results: 100% of sections in group A and 93.3% of sections in group B were adequate for diagnosis (p-value 0.150).
Conclusion: The study recommends refined mineral oil as a biofriendly and effective xylene substitute in deparaffinization of tissue sections.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.5005/jp-journals-10024-1314 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Characteristics and paleoclimate significance of authigenic ferrimagnetic minerals in the Xuancheng red earth, southern China.
Sci Rep
January 2025
School of Earth Sciences, China University of Geosciences, Wuhan, 430074, Hubei, China.
Soil magnetic records in Quaternary red earth (QRE) deposits contain a valuable record of paleoclimate information, providing insights into controls on Earth's climate system in the past and potentially helping to predict its response to perturbations in the future. Here, analysis of the environmental magnetism and mineralogy of the Xuancheng QRE (Anhui Province, South China) shows that magnetic variation was strongly linked to production of authigenic ferrimagnetic minerals such as maghemite. Fine-grained maghemite formed during the weathering-related transformation of iron-bearing illite to vermiculite, generating aggregates of vermiculite or mixed-layer illite-vermiculite.
View Article and Find Full Text PDFIndonesia's country-specific CO emission factor based on gas fuels for greenhouse gas inventory in the energy sector.
Environ Pollut
January 2025
Testing Center for Oil and Gas LEMIGAS, Ministry of Energy and Mineral Resources, Jakarta Selatan 12230 Indonesia.
Indonesia currently calculates CO emissions from gas fuels using Tier 1 emission factors adopted from the Intergovernmental Panel on Climate Change (IPCC). However, this method may not accurately capture the country's specific emission characteristics. To address this, this study aims to derive country-specific CO emission factors for gas fuels, including liquefied petroleum gas (LPG), liquefied gas for vehicles (LGV), natural gas (NG), and liquefied natural gas (LNG), by analyzing fuel samples collected nationwide.
View Article and Find Full Text PDFLead/cadmium impacts on zeolite-tobermorite: Nutrient release and sediment stability.
Environ Pollut
January 2025
Department of Mineral Processing Engineering, Taiyuan University of Technology, Taiyuan, 030024, China.
To realize the comprehensive utilization of large amounts of high-ash coal slime and comprehensively understand the excellent performance of nutrient release and lead and cadmium adsorption of high-ash coal slime silicon composite materials, green and safe mild hydrothermal conditions (200 °C) were used to prepare the rich-rich coal slime. Zeolite/tobermorite composites (Z-TOBs) were used in this study. Batch adsorption tests and repeated extraction tests were used to determine whether silicon, potassium, and calcium nutrients of Z-TOBs have sustained release properties and are affected by pH.
View Article and Find Full Text PDFStudy of Adhesion-Cohesive Interactions of Modified Bitumen Compositions.
Polymers (Basel)
January 2025
Department of Chemistry and Chemical Technology, Manash Kozybayev North Kazakhstan University, Petropavlovsk 150000, Kazakhstan.
The aim of the work was to study the effect of additive concentration on changes in the adhesive and cohesive strength of bitumen. To evaluate the effectiveness of modifiers in the composition of binary and triple bitumen systems in relation to mineral fillers of two grades, the method of determination of the adhesive efficiency and thermodynamic calculations of adhesion and cohesion work were used. The following compounds were used as additives: synthesized from the oil refining waste and (waste sealing liquid).
View Article and Find Full Text PDFA Solar-Heated Phase Change Composite Fiber with a Core-Shell Structure for the Recovery of Highly Viscous Crude Oil.
Polymers (Basel)
January 2025
Binzhou Institute of Technology, Weiqiao-UCAS Science and Technology Park, Binzhou 256606, China.
Due to the high viscosity and low fluidity of viscous crude oil, how to effectively recover spilled crude oil is still a major global challenge. Although solar thermal absorbers have made significant progress in accelerating oil recovery, its practical application is largely restricted by the variability of solar radiation intensity, which is influenced by external environmental factors. To address this issue, this study created a new composite fiber that not only possesses solar energy conversion and storage capabilities but also facilitates crude oil removal.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!