J Microbiol Methods
Division Veterinary Public Health, Institute for Risk Assessment Sciences, Utrecht University, The Netherlands.
Published: October 2013
A real time quantitative PCR combined with propidium monoazide (PMA) treatment of samples was implemented to quantify live C. jejuni, C. coli and C. lari on broiler chicken carcasses at selected processing steps in the slaughterhouse. The samples were enumerated by culture for comparison. The Campylobacter counts determined with the PMA-qPCR and the culture method were not concordant. We conclude that the qPCR combined with PMA treatment of the samples did not fully reduce the signal from dead cells.
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http://dx.doi.org/10.1016/j.mimet.2013.06.003 | DOI Listing |
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