Zinc plays a critical role in a diverse array of biochemical processes. However, an excess of zinc is deleterious to cells; therefore, cells require finely tuned homeostatic mechanisms to balance the uptake and the storage of zinc. There is also increasing evidence supporting the importance of zinc during infection. To understand better how Paracoccidioides adapts to zinc deprivation, we compared the two-dimensional (2D) gel protein profile of yeast cells during zinc starvation to yeast cells grown in a zinc rich condition. Protein spots were selected for comparative analysis based on the protein staining intensity, as determined by image analysis. In response to zinc deprivation, a total of 423 out of 845 protein spots showed a significant change in abundance. Quantitative RT-qPCR analysis of RNA from Paracoccidioides grown under zinc restricted conditions validated the correlation between the differentially regulated proteins and transcripts. According to the proteomic data, zinc deficiency may be a stressor to Paracoccidioides, as suggested by the upregulation of a number of proteins related to stress response, cell rescue, and virulence. Other process induced by zinc deprivation included gluconeogenesis. Conversely, the methylcitrate cycle was downregulated. Overall, the results indicate a remodelling of the Paracoccidioides response to the probable oxidative stress induced during zinc deprivation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.funbio.2013.04.004 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Exploiting the fitness cost of metallo-β-lactamase expression can overcome antibiotic resistance in bacterial pathogens.
Nat Microbiol
January 2025
Institute of Infectious Disease Research, McMaster University, Hamilton, Ontario, Canada.
Carbapenems are last-resort antibiotics for treating bacterial infections. The widespread acquisition of metallo-β-lactamases, such as VIM-2, contributes to the emergence of carbapenem-resistant pathogens, and currently, no metallo-β-lactamase inhibitors are available in the clinic. Here we show that bacteria expressing VIM-2 have impaired growth in zinc-deprived environments, including human serum and murine infection models.
View Article and Find Full Text PDFEarly growth response-1, a dynamic conduit in cardiovascular disease.
Front Cardiovasc Med
November 2024
Vascular Biology and Translational Research, Department of Pathology, Faculty of Medicine and Health, School of Biomedical Sciences, University of New South Wales, Sydney, NSW, Australia.
The transcription factor, early growth response-1 (Egr-1) is the product of a prototypic immediate-early gene that plays an integral role in the pathogenesis of multiple cardiovascular diseases. Egr-1 has been linked with atherogenesis, myocardial ischemia-reperfusion injury, cardiac fibrosis and heart failure. Egr-1 expression is triggered by a host of factors including cytokines, hormones, growth factors, hyperglycaemia, biomechanical forces and oxygen deprivation.
View Article and Find Full Text PDFReplacing sulfate with hydroxychloride sources of trace minerals modulated the growth performance and plasma indicators of inflammation and energy metabolism in beef heifers during periods of feed restriction and adaptation to a high-starch diet.
J Anim Sci
January 2024
Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu, 8618-970, Brazil.
This study evaluated the effects of different sources (sulfate vs. hydroxychloride) of Cu, Mn, and Zn during feed restriction and a high-starch diet on heifer growth performance. On day 0, Nelore heifers (n = 40) were stratified by body weight (BW = 238 ± 38 kg) and age (21 ± 1 mo), and individually allocated into 1 of the 40 drylot pens.
View Article and Find Full Text PDFmiRNAs regulate the metabolic adaptation of Paracoccidioides brasiliensis during copper deprivation.
Microbes Infect
November 2024
Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, 74690-900, Brazil. Electronic address:
Article Synopsis
- - Copper is vital for cellular functions like detoxifying harmful molecules and energy production, but during infections, the body limits its availability to hinder pathogens.
- - The study investigates the role of miRNAs (a type of regulatory molecule) in the response of the fungus P. brasiliensis to low copper levels, complementing earlier findings on iron and zinc.
- - Using RNA sequencing, researchers identified 14 miRNAs that change expression during copper scarcity, with implications for processes like oxidative stress and cell structure adaptation, indicating miRNAs play a key role in the fungus’s metabolic adjustments.
Functional and structural analyses of IMP-27 metallo-β-lactamase: evolution of IMP-type enzymes to overcome Zn(II) deprivation.
Microbiol Spectr
November 2024
Department of Applied Life Sciences, Niigata University of Pharmacy and Applied Life Sciences, Niigata, Japan.
IMP-type metallo-β-lactamases are di-Zn(II) enzymes that can inactivate a wide range of bicyclic β-lactam agents used in clinical practice. IMP-27 shares 82% amino acid sequence identity with IMP-1, the first IMP-type enzyme identified. Herein, we conducted structural determination, kinetic, and chelating agent resistance analyses of IMP-27.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!