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Lymphatic collection and cell isolation from mouse models for multiomic profiling.
Nat Protoc
January 2025
Department of Molecular Metabolism, Harvard T.H. Chan School of Public Health, Boston, MA, USA.
Premetastatic cancer cells often spread from the primary lesion through the lymphatic vasculature and, clinically, the presence or absence of lymph node metastases impacts treatment decisions. However, little is known about cancer progression via the lymphatic system or of the effect that the lymphatic environment has on cancer progression. This is due, in part, to the technical challenge of studying lymphatic vessels and collecting lymph fluid.
View Article and Find Full Text PDFA National Assessment of Pediatric Surgical Critical Care Fellowship Structure: Defining the Training Experience.
J Pediatr Surg
December 2024
University of Michigan Department of Surgery, Division of Pediatric Surgery, 1540 East Hospital Drive Ann Arbor, MI 48109-4211, USA. Electronic address:
Background: Pediatric Surgical Critical Care (PSCC) is a unique specialty incorporating fundamental principles of surgical, neonatal, and pediatric critical care. This study aims to characterize the current landscape of PSCC training to identify opportunities for educational standardization and improvement.
Methods: An anonymous electronic survey-based assessment was distributed to the program directors (PDs) of all current ACGME-accredited PSCC fellowships (n = 14).
Barriers to accessing mental health resources as perceived by surgical compared to medical trainees.
Am J Surg
December 2024
Geisinger Medical Center, Department of General Surgery, 100 N Academy Ave, Danville, PA, USA.
Introduction: Despite myriad wellness programs, training demands restrict resident access. This study aimed to evaluate barriers to accessing mental health resources(MHR) and determine if differences exist between medical(MT) and surgical trainees(ST).
Methods: A cross-sectional survey was performed in one health system assessing awareness of and barriers to accessing MHR.
Introduction: There has been limited research on predicting the functional prognosis of patients with non-surgical intracerebral hemorrhage (ICH) from the acute stage. The aim of this study is to develop a risk prediction model for the natural course in patients with non-surgical ICH and to evaluate its performance using a multicenter hospital-based prospective study of stroke patients in Japan.
Methods: We consecutively registered a total of 1,017 patients with acute ICH (mean age, 68 years) who underwent conservative treatment and followed them up for 3 months.
The Role of [F]F-FDG PET/CT for Predicting Histology and Prognosis in Patients with Thymic Lesions.
Mol Diagn Ther
January 2025
Thoracic Surgery and Lung Transplantation, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, 20122, Milan, Italy.
Objectives: To investigate whether 18F-fluorodeoxyglucose positron emission tomography-computed tomography ([F]F-FDG PET/CT) metabolic parameters were associated with histology and to assess their prognostic role in patients with thymic lesions.
Patients And Methods: In total, 116 patients (49/67 M/F; mean age 59.5 years) who underwent preoperative [F]F-FDG PET/CT and thymectomy from 2012 to 2022 were retrospectively analyzed.
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