AI Article Synopsis
- A new enzyme called N-acetylglucosamine-1-phosphate pyrophosphorylase was discovered in Campylobacter jejuni NCTC 11168, which shows a high ability to work with various substrates.
- Systematic studies demonstrated that the enzyme can efficiently catalyze the formation of sugar nucleotides, achieving yields of up to 60% for some sugars and around 20% for others.
- The research led to the synthesis and detailed characterization of three new sugar nucleotide analogs using this enzyme on a large scale.
Article Abstract
A novel N-acetylglucosamine-1-phosphate pyrophosphorylase was identified from Campylobacter jejuni NCTC 11168. An unprecedented degree of substrate promiscuity has been revealed by systematic studies on its substrate specificities towards sugar-1-P and NTP. The yields of the synthetic reaction of seven kinds of sugar nucleotides catalyzed by the enzyme were up to 60%. In addition, the yields of the other nine were around 20%. With this enzyme, three novel sugar nucleotide analogs were synthesized on a preparative scale and well characterized.
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| http://dx.doi.org/10.1016/j.bmcl.2013.06.003 | DOI Listing |
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