Introduction: When developing tissue engineered solutions for existing clinical problems, cell seeding strategies should be optimized for desired cell distribution within matrices. The purpose of this investigation was to compare the effects of different static cell seeding methods and subsequent static cell culture for up to 12 days with regard to seeding efficiency and resulting cellular distribution in acellular dermis.
Materials And Methods: The seeding methods tested were surface seeding of both unmodified and mechanically incised dermis, syringe injection of cell suspension, application of low-pressure and use of an ultrasonic bath to remove trapped air. The effect of "platelet derived growth factor" (PDGF) on surface seeding and low pressure seeding was also investigated. Scaffolds were incubated for up to 12 days and were histologically examined at days 0, 4, 8 and 12 for cell distribution and infiltration depth. The metabolic activity of the cells was quantified with the MTT assay at the same time points.
Results: The 50 ml syringe degassing procedure produced the best results in terms of seeding efficiency, cell distribution, penetration depth and metabolic activity within the measured time frame. The injection and ultrasonic bath methods produced the lowest seeding efficiency. The incision method and the 20 ml syringe degassing procedure produced results that were not significantly different to those obtained with a standard static seeding method.
Conclusion: We postulate that air in the pores of the human acellular dermis (hAD) hinders cell seeding and subsequent infiltration. We achieved the highest seeding efficiency, homogeneity, infiltration depth and cell growth within the 12 day static culturing period by degassing the dermis using low- pressure created by a 50 ml syringe. We conclude that this method to eliminate trapped air provides the most effective method to seed cells and to allow cell proliferation in a natural scaffold.
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http://dx.doi.org/10.1186/1475-925X-12-55 | DOI Listing |
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January 2025
Chemical and Petroleum Engineering Department, College of Engineering, United Arab Emirates University, PO Box 15551, Al Ain, United Arab Emirates. Electronic address:
In this study, the role of a transition metal complex in improving hydrolysis efficiency during nanocellulose production was analysed. Cellulose nanocrystals (CNCs) were extracted from date seeds by incorporating a copper metal complex during HCl hydrolysis. In contrast to traditional HCl hydrolysis at moderate conditions, which yielded only microcrystalline cellulose (MCC), this approach resulted in the extraction of CNCs with a 10 % improved yield compared to MCC.
View Article and Find Full Text PDFEnviron Sci Pollut Res Int
January 2025
Nano Photocatalysis Lab., Department of Chemistry, College of Science, Shiraz University, Shiraz, 7194684795, I.R. of Iran.
Due to the industry's rapid growth, the presence of organic pollutants, especially antibiotics, in water and wastewater resources is the main concern for wildlife and human health. Therefore, these days, a significant challenge is developing an efficient, sustainable, and eco-friendly photocatalyst. Natural biological models have numerous advantages compared to artificial model materials.
View Article and Find Full Text PDFTalanta
December 2024
Department of Chemistry, Government College University, Lahore, Pakistan. Electronic address:
The current research focused on extraction optimization of bioactive compounds from Strychnos potatorum seeds (SPs) using an eco-friendly glycerol-sodium acetate based deep eutectic solvent (DES). The optimization was accomplished using response surface methodology (RSM) and artificial neural networking (ANN). The independent variables included shaking time (A), temperature (B), and solvent-to-feed ratio (C), and the responses were the extraction yield, total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity (DPPH), and antidiabetic activity (α-amylase inhibitory activity).
View Article and Find Full Text PDFAlzheimers Dement
December 2024
Stark Neurosciences Research Institute, Indiana University School of Medicine, Indianapolis, IN, USA.
Alzheimers Dement
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University of Malaga/CIBERNED/IBIMA, Málaga, Spain.
Background: Alzheimer's Disease (AD) is a neurodegenerative proteinopathy in which Aβ can misfold and aggregate into seeds that structurally corrupt native proteins, mimicking a prion-like process. These amyloid aggregation and propagation processes are influenced by three factors: the origin of the Aβ seed, time of incubation and host. However, the mechanism underlying the differential effect of each factor is poorly known.
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