CAPRICE (CPC) and CAPRICE-like (CPL) myeloblastosis (MYB) family members [including TRYPTICHON (TRY) and ENHANCER OF TRYPTICHON AND CAPRICE (ETC)] of Arabidopsis thaliana encode R3-type MYB transcription factors that promote root hair differentiation and inhibit trichome formation in a redundant manner. Previously, we reported that the CPL3 gene affects flowering. The cpl3 mutant plants flower earlier and with fewer leaves than the wild type. In this study, we show that mutations in CPC or TRY delay flowering of cpl3 plants. A mutation in ETC1 did not further delay flowering but reduced plant size. Our study provides insight into the regulation of flowering time by the CPC-like MYB gene family.
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http://dx.doi.org/10.1016/j.jplph.2013.05.013 | DOI Listing |
Nat Plants
May 2019
Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.
Post-transcriptional gene silencing (PTGS) is a major mechanism regulating gene expression in higher eukaryotes. To identify novel players in PTGS, a forward genetics screen was performed on an Arabidopsis thaliana line overexpressing a strong growth-repressive gene, ETHYLENE RESPONSE FACTOR6 (ERF6). We identified six independent ethyl-methanesulfonate mutants rescuing the dwarfism of ERF6-overexpressing plants as a result of transgene silencing.
View Article and Find Full Text PDFNat Genet
April 2019
Department of Molecular Biology, Princeton University, Princeton, NJ, USA.
Photosynthetic organisms provide food and energy for nearly all life on Earth, yet half of their protein-coding genes remain uncharacterized. Characterization of these genes could be greatly accelerated by new genetic resources for unicellular organisms. Here we generated a genome-wide, indexed library of mapped insertion mutants for the unicellular alga Chlamydomonas reinhardtii.
View Article and Find Full Text PDFPlant J
September 2017
Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian, 361102, China.
CPSF100 is a core component of the cleavage and polyadenylation specificity factor (CPSF) complex for 3'-end formation of mRNA, but it still has no clear functional assignment. CPSF100 was reported to play a role in RNA silencing and promote flowering in Arabidopsis. However, the molecular mechanisms underlying these phenomena are not fully understood.
View Article and Find Full Text PDFPlant Sci
December 2015
Graduate School of Biosphere Sciences, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima 739-8528, Japan. Electronic address:
CAPRICE (CPC) and six additional CPC family genes encode R3-type MYB transcription factors involved in epidermal cell fate determination, including Arabidopsis root hair and trichome differentiation. Previously, we reported that the CPC and CPC family genes TRIPTYCHON (TRY) and CAPRICE LIKE MYB3 (CPL3) also affect flowering time. The cpl3 mutant plants flower earlier, with fewer but larger leaves, than do wild type plants, and mutations in CPC or TRY delay flowering in the cpl3 mutant.
View Article and Find Full Text PDFJ Plant Physiol
November 2013
Interdisciplinary Research Organization, University of Miyazaki, 1-1, Gakuen Kibanadai-Nishi, Miyazaki 889-2192, Japan. Electronic address:
CAPRICE (CPC) and CAPRICE-like (CPL) myeloblastosis (MYB) family members [including TRYPTICHON (TRY) and ENHANCER OF TRYPTICHON AND CAPRICE (ETC)] of Arabidopsis thaliana encode R3-type MYB transcription factors that promote root hair differentiation and inhibit trichome formation in a redundant manner. Previously, we reported that the CPL3 gene affects flowering. The cpl3 mutant plants flower earlier and with fewer leaves than the wild type.
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