The complete mitogenome sequence of Acrossocheilus monticolus, which is endemic to China, was determined using long PCR reactions. The genome is 16,599 bp in length, including 13 typical vertebrate protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and a control region. Except for eight tRNA and ND6 genes, all other mitochondrial genes are encoded on the heavy strand. The gene order and composition of A. monticolus was similar to that of most other vertebrates. The descending order of the base composition on heavy strand was 31.4% A, 28.2% C, 24.5% T, 15.8% G, with a relatively lower level of G and a slight AT bias of 55.9%. The codon usage followed the typical vertebrate mitochondrial pattern (ATG or GTG for start codon and TAA or TAG for stop codon). There are 7 regions of gene overlap totaling 23 bp and 13 intergenic spacer regions totaling 69 bp.
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Chromosome-scale genome assemblies of sexually dimorphic male and female Acrossocheilus fasciatus.
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Background: The liver-expressed antimicrobial peptide 2 (LEAP2) plays a pivotal role in the host's immune response against pathogenic microorganisms. Numerous such antimicrobial peptides have recently been shown to mitigate infection risk in fish, and studying those harboured by the economically important fish Acrossocheilus fasciatus is imperative for enhancing its immune responses against pathogenic microorganisms. In this study, we cloned and sequenced LEAP2 cDNA from A.
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