We proved the feasibility of using a microfluidic chip to culture diatom Bacillaria paradoxa, and analyzed the gliding characteristics of its self-organized colony in detail. The optimal cultivation parameters of B. paradoxa for the designed chip made with polydimethylsiloxane are as follows: the preferable cells injecting rate for keeping the cells alive is 0.2 mL/h, the initial cell density for fast reproduction is 5.5 × 10(4) cells/mL, and the optimal replacement period of culture medium is 4 days. B. paradoxa tends to form a colony during their growth, and the colony can glide with a steady period of 29 ± 3 s along its axial direction in a constant stream, the amplitude of the colony will not decay (e.g., 24 μm of two-cell colony at 1.1 mm/s flow rate), and the colony rapidly adjusts its direction of gliding to the direction of water flow. The successful culture of diatoms on a microfluidic platform may be used for biosensing chips and the creation of gasoline-producing diatom solar panels.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s00284-013-0413-7 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
First investigation of the temporal distribution of neurotoxin β-N-methylamino-L-alanine (BMAA) and the candidate causative microalgae along the South Sea Coast of Korea.
J Hazard Mater
October 2024
Department of Marine Environmental Sciences & Institute of Marine Environmental Sciences, Chungnam National University, Daejeon 34134, Republic of Korea; Department of Earth, Environmental & Space Sciences, Chungnam National University, Daejeon 34134, Republic of Korea. Electronic address:
Article Synopsis
- The study explored the presence and distribution of the neurotoxin β-N-methylamino-L-alanine (BMAA) and its related compounds in phytoplankton and mussels along the South Sea Coast of Korea throughout 2021.
- BMAA concentrations were found to peak in phytoplankton in November and in mussels in December, with a notable lag between the two, while DAB was present in phytoplankton but not in mussels.
- The research identified specific diatom genera as potential sources of BMAA and indicated an inverse relationship between chlorophyll-a and BMAA levels in mussels, contributing to understanding biotoxins in marine environments.
Shifts in species dominance related to spatial assemblages and variation in environmental parameters in a tropical mangrove estuary.
Mar Environ Res
October 2023
Department of Oceanography and Coastal Sciences, Louisiana State University, Baton Rouge, LA, 70803, USA.
A shift in species dominance in the mangrove microalgae community in Cross River Estuary System (CRES), Nigeria, was carried out during the wet season to highlight the microalgae structure, identify potential indicators, and evaluate the water quality variation. Plankton samples were collected at sixteen selected sampling sites along the river. Diatoms showed the highest number of species (240 species), contributing more than 70% of the total microalgae abundance.
View Article and Find Full Text PDFComparative Analysis of Bacillariophyceae Chloroplast Genomes Uncovers Extensive Genome Rearrangements Associated with Speciation.
Int J Environ Res Public Health
August 2022
CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
The Bacillariophyceae is a species-rich, ecologically significant class of Bacillariophyta. Despite their critical importance in marine ecosystems as primary producers and in the development of harmful algal blooms (HABs), taxonomic research on Bacillariophyceae species has been hindered because of their limited morphological features, plasticity of morphologies, and the low resolution of common molecular markers. Hence molecular markers with improved resolution are urgently needed.
View Article and Find Full Text PDFShort-term changes in algal blooms and phytoplankton community after the passage of Super Typhoon Lekima in a temperate and inner sea (Bohai Sea) in China.
Ecotoxicol Environ Saf
March 2022
Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
Extensive multi-species harmful algal blooms (HABs) were triggered by Super Typhoon Lekima in Laizhou Bay (Bohai Sea) in August 2019. After conducting two field cruises before and after the typhoon passage, we employed both high-performance liquid chromatography (HPLC)-pigment and microscopic methods to study the changes in the phytoplankton community and biomass. Following the passage of Lekima, the average surface salinity decreased, while dissolved inorganic nitrogen and dissolved silicate concentrations increased in the study area.
View Article and Find Full Text PDFThe polar night shift: seasonal dynamics and drivers of Arctic Ocean microbiomes revealed by autonomous sampling.
ISME Commun
December 2021
Deep-Sea Ecology and Technology, Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, Bremerhaven, Germany.
The Arctic Ocean features extreme seasonal differences in daylight, temperature, ice cover, and mixed layer depth. However, the diversity and ecology of microbes across these contrasting environmental conditions remain enigmatic. Here, using autonomous samplers and sensors deployed at two mooring sites, we portray an annual cycle of microbial diversity, nutrient concentrations and physical oceanography in the major hydrographic regimes of the Fram Strait.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!