The protective effect of osmolytes on the thermal denaturation and aggregation of Pelodiscus sinensis muscle creatine kinase (PSCK) was investigated by a combination of spectroscopic methods and thermodynamic analysis. Our results demonstrated that the addition of osmolytes, such as glycine and proline, could prevent thermal denaturation and aggregation of PSCK in a concentration-dependent manner. When the concentration of glycine and proline increased in the denatured system, the relative activation was significantly enhanced; meanwhile, the aggregation of PSCK during thermal denaturation was decreased. Spectrofluorometer results showed that glycine and proline significantly decreased the tertiary structural changes of PSCK and that thermal denaturation directly induced PSCK aggregation. In addition, we also built the 3D structure of PSCK and osmolytes by homology models. The results of computational docking simulations showed that the docking energy was relatively low and that the clustering groups were spread to the surface of PSCK, indicating that osmolytes directly protect the surface of the protein. P. sinensis are poikilothermic and quite sensitive to the change of ambient temperature; however, there were few studies on the thermal denaturation of reptile CK. Our study provides important insight into the protective effects of osmolytes on thermal denaturation and aggregation of PSCK.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ijbiomac.2013.06.015 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Heterologous expression of calcium-independent mesophilic α-amylase from Priestia megaterium: Immobilization on genipin-modified multi-walled carbon nanotubes and silica supports to enhance thermostability and catalytic activity.
Bioorg Chem
January 2025
Department of Bioengineering, Gebze Technical University, 41400, Gebze, Kocaeli, Türkiye; BAUZYME Biotechnology Co., Gebze Technical University Technopark, 41400, Gebze, Kocaeli, Türkiye. Electronic address:
α-Amylases, constituting a significant share of the enzyme market, are mainly synthesized by the genus Bacillus. Enzymes tailored for specific industrial applications are needed to meet the growing demand across a range of industries, and thus finding new amylases and optimizing the ones that already exist are extremely important. This study reports the successful expression, characterization and immobilization of P.
View Article and Find Full Text PDFOptimized microwave-assisted extraction and characterization of spray dried Luffa aegyptiaca nanomucilage: Physicochemical properties, biological activities, and anticancer efficacy against MCF-7 human breast cancer cells.
Int J Biol Macromol
January 2025
Department of Food Technology and Nutrition, Lovely Professional University, Phagwara 144411, India. Electronic address:
Microwave-assisted extraction conditions were optimized using response surface methodology to evaluate the effects of extraction parameters on the yield and carbohydrate content of Luffa aegyptiaca mucilage. Extraction at 540 W for 2 min with a 1:20 (g/mL) was determined as the optimal parameter, resulting in a maximum yield of 5.90 % (w/w) with 63 % carbohydrate content consisting of glucose, galactose, maltose, mannose, and galacturonic acid, with structural linkages of β (1 → 4) and β (1 → 6) glycosidic bonds.
View Article and Find Full Text PDFProtein thermal stability in the undergraduate biochemistry laboratory: Exploring protein thermal stability with yeast alcohol dehydrogenase.
Biochem Mol Biol Educ
January 2025
Department of Chemistry and Biochemistry, Miami University, Oxford, Ohio, USA.
We created a novel laboratory experience where undergraduate students explore the techniques used to study protein misfolding, unfolding, and aggregation. Despite the importance of protein misfolding and aggregation diseases, protein unfolding is not typically explored in undergraduate biochemistry laboratory classes. Yeast alcohol dehydrogenase (YADH) is used in the undergraduate biochemistry laboratory course at Miami University as the model system to explore protein overexpression and purification, bioinformatics, and enzyme characterization.
View Article and Find Full Text PDFImpact of thermal denaturation on renal volume reduction after partial nephrectomy using soft coagulation hemostasis.
Sci Rep
January 2025
Department of Urology, NTT Medical Center Tokyo, 5-9-22 Higashigotanda, Shinagawa-ku, Tokyo, 141-8625, Japan.
Partial nephrectomy has become the gold standard treatment for small renal masses. This study aimed to assess the impact of soft coagulation hemostasis on parenchymal volume reduction of the operated kidney after an open partial nephrectomy. We retrospectively reviewed 94 patients with small renal tumors who underwent open partial nephrectomy with soft-coagulation hemostasis at our institution.
View Article and Find Full Text PDFEvaluation of Thermal Liquid Biopsy Analysis of Saliva and Blood Plasma Specimens as a Novel Diagnostic Modality in Head and Neck Cancer.
Cancers (Basel)
December 2024
UofL Health-Brown Cancer Center, Department of Medicine, University of Louisville, Louisville, KY 40202, USA.
Background: Over the past decade, saliva-based liquid biopsies have emerged as promising tools for the early diagnosis, prognosis, and monitoring of cancer, particularly in high-risk populations. However, challenges persist because of low concentrations and variable modifications of biomarkers linked to tumor development when compared to normal salivary components.
Methods: This study explores the application of differential scanning calorimetry (DSC)-based thermal liquid biopsy (TLB) for analyzing saliva and blood plasma samples from head and neck cancer (HNC) patients.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!