AI Article Synopsis
- - The study investigates how vitamin B12 deficiency affects DNA methylation in women of childbearing age, specifically looking at blood samples from those with low vitamin B12 levels (plasma <148 pmol/L).
- - Results showed that DNA from vitamin B12-deficient women had significantly lower deoxycytidine methylation levels and higher methyl-acceptance in coagulated blood samples compared to B12-replete women.
- - Interestingly, no significant differences in DNA methylation were found in uncoagulated blood samples, indicating that the method of sample collection plays a crucial role in the results of epigenetic studies.
Article Abstract
Vitamin B12, a co-factor in methyl-group transfer, is important in maintaining DNA (deoxycytidine) methylation. Using two independent assays we examined the effect of vitamin B12-deficiency (plasma vitamin B12<148 pmol/L) on DNA methylation in women of childbearing age. Coagulated blood clot DNA from vitamin B12-deficient women had significantly (p<0.001) lower percentage deoxycytidine methylation (3.23±0.66%; n = 248) and greater [3 H]methyl-acceptance (42,859±9,699 cpm; n = 17) than DNA from B12-replete women (4.44±0.18%; n = 128 and 26,049±2,814 cpm; n = 11) [correlation between assays: r = -0.8538; p<0.001; n = 28]. In contrast, uncoagulated EDTA-blood cell pellet DNA from vitamin B12-deficient and B12-replete women exhibited similar percentage methylation (4.45±0.15%; n = 77 vs. 4.47±0.15%; n = 47) and [3 H]methyl-acceptance (27,378±4,094 cpm; n = 17 vs. 26,610±2,292 cpm; n = 11). Therefore, in simultaneously collected paired blood samples, vitamin B12-deficiency was associated with decreased DNA methylation only in coagulated samples. These findings highlight the importance of sample collection methods in epigenetic studies, and the potential impact biological processes can have on DNA methylation during collection.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3681792 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0065241 | PLOS |
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