AI Article Synopsis
- This study examines the yield of connective-tissue progenitors from different areas of the iliac crest (anterior vs posterior) to improve stem cell applications in therapy.
- Researchers collected bone marrow from 22 patients and found the posterior iliac crest yielded 1.6 times more colony-founding progenitors compared to the anterior, although the cell viability and differentiation abilities were similar for both.
- The findings suggest that harvesting bone marrow from the posterior iliac crest is more effective for obtaining progenitors, which could enhance the clinical use of mesenchymal stem cells.
Article Abstract
Background: The clinical application of freshly isolated connective-tissue progenitors, as well as the potential preparation of culture-expanded mesenchymal stem cell populations for therapeutic applications, will benefit from clinical methods that maximize the yield of the starting population. We compared the number of cells, concentration, and prevalence of colony-founding connective-tissue progenitors from the anterior and posterior iliac crest. In addition, we compared the expansion kinetics and multilineage differentiation potential of their culture-expanded progeny when processed to form mesenchymal stem cells.
Methods: Marrow aspirate was collected from both the anterior and posterior iliac crest of twenty-two patients. The concentration and prevalence of colony-founding connective-tissue progenitors were estimated with use of a colony formation assay. The expansion kinetics and multilineage differentiation potential of the culture-expanded mesenchymal stem cell populations derived from these starting samples were compared.
Results: The yield of colony-founding connective-tissue progenitors was 1.6 times greater in the posterior compared with the anterior iliac crest. No differences were found with respect to the viability, phenotype, expansion kinetics, or multilineage differentiation potential of mesenchymal stem cell populations derived from these two sites.
Conclusions: The concentration and yield of colony-founding connective-tissue progenitors were greater when aspirate was obtained from the posterior compared with the anterior iliac crest, whereas the biological potential of the cells derived from these sites appeared comparable.
Clinical Relevance: The harvesting of bone marrow from the posterior iliac crest appears to be preferred, as it provided a modestly higher concentration of colony-founding connective-tissue progenitors than comparable aspirate from the anterior iliac crest.
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| http://dx.doi.org/10.2106/JBJS.L.00429 | DOI Listing |
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