Estimation of sertraline by chromatographic (HPLC-UV273 nm) technique under hydrolytic stress conditions.

Purpose: In this paper, simple, specific and accurate RP-HPLC method was developed in order to study decomposition of sertraline (SRT) under the hydrolytic stress conditions (acid, neutral, alkaline and oxidative).

Materials And Methods: The best separation of SRT and its degradation products were achieved on reverse phase LiChoCART with Purospher (RP-18e) column. The mobile phase was composed of methanol/water (75:25, v/v). The detection wavelength was 273 nm. The method was validated and response was found to be linear in the drug concentration range of 10-200 μg ml-1 with correlation coefficient of 0.998.

Results: The RSD values for intra- and inter-day precision were < 0.65 and < 0.72%, respectively. Employing RP-HPLC method, degradation products were detected in the exposed samples.

Conclusion: IT WAS FOUND THAT THE SUSCEPTIBILITY OF SRT TO HYDROLYTIC DECOMPOSITION INCREASED IN FOLLOWING MANNER: Neutral condition < alkaline condition < acid condition < oxidative condition.