AI Article Synopsis
- Agouti-related protein (AgRP) is a key neuropeptide that regulates feeding in both mammals and fish, and this study focused on the cloning of its full-length cDNA sequence in the cyprinid fish Schizothorax prenanti.
- The AgRP gene, which encodes a 126-amino acid protein, was found to be highly expressed in the brain and was present in embryos and unfertilized eggs.
- Research indicated that AgRP expression significantly increased during short-term fasting (3-4 hours) in fish, while long-term fasting (14 days) did not affect its mRNA levels, suggesting AgRP plays a role in short-term feeding regulation and other physiological functions.
Article Abstract
Agouti-related protein (AgRP) is an important neuropeptide involved in the regulation of feeding in both mammals and fish. In this study, we have cloned the full-length cDNA sequence for AgRP in a cyprinid fish (Schizothorax prenanti). The AgRP gene, encoding 126-amino acids, was strongly expressed in the brain. The AgRP gene was detected in embryos at developmental stages. Further, its mRNA was detectable in unfertilized eggs. An experiment was conducted to determine the expression profile of AgRP during short-term and long-term fasting of the hypothalamus. The expression level of AgRP in unfed fish was significantly increased at 3 and 4h post-fasting than in fed fish but did not affect AgRP mRNA expression after 14 days fasting. Overall, our results suggest that AgRP is a conserved peptide that might be involved in the regulation of short-term feeding and other physiological function in Schizothorax prenanti.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.gene.2013.06.003 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!