Correlative microscopy for phylogenetic and ultrastructural characterization of microbial communities.

Environ Microbiol Rep

Lawrence Berkeley National Laboratory, Life Sciences Division, Berkeley, CA, USA. Department of Earth and Planetary Science Department of Environmental Science, Policy and Management, University of California, Berkeley, Berkeley, CA, USA. Centre for Microscopy and Microanalysis (CMM), The University of Queensland, St. Lucia, Queensland, Australia.

Published: February 2012

AI Article Synopsis

  • TEM allows for detailed examination of cell structure in microbial communities, while molecular surveys give insights into their evolutionary relationships.
  • This study introduces a method that combines fluorescence in situ hybridization (FISH) with standard or cryogenic TEM techniques.
  • This approach aims to enhance our knowledge of how microbial communities are structured and how they function in their natural environments.

Article Abstract

Transmission electron microscopy (TEM) can provide ultrastructural information for cells in microbial community samples and phylogenetic information can be recovered via molecular surveys. Here we report an approach to link these data sets by coupling fluorescence in situ hybridization (FISH) with either conventional biological or cryogenic TEM. The method could fundamentally improve our understanding of the organization and functioning of microbial communities in natural systems.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4444221PMC
http://dx.doi.org/10.1111/j.1758-2229.2011.00275.xDOI Listing

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