In embryonic stem (ES) cells and in early mouse embryos, the transcription factor Oct4 is an essential regulator of pluripotency. Oct4 transcriptional targets have been described in ES cell lines; however, the molecular mechanisms by which Oct4 regulates establishment of pluripotency in the epiblast (EPI) have not been fully elucidated. Here, we show that neither maternal nor zygotic Oct4 is required for the formation of EPI cells in the blastocyst. Rather, Oct4 is first required for development of the primitive endoderm (PE), an extraembryonic lineage. EPI cells promote PE fate in neighboring cells by secreting Fgf4, and Oct4 is required for expression of Fgf4, but we show that Oct4 promotes PE development cell-autonomously, downstream of Fgf4 and Mapk. Finally, we show that Oct4 is required for the expression of multiple EPI and PE genes as well as multiple metabolic pathways essential for the continued growth of the preimplantation embryo.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4076445 | PMC |
| http://dx.doi.org/10.1016/j.devcel.2013.05.004 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Research note: Harnessing the potential of chicken blastodermal cells as a new frontier in poultry science and biotechnology - Establishment of embryonic stem cells and their differentiability towards Schwann cell-like cell lineages.
Poult Sci
December 2024
Department of Reproductive Biotechnology and Cryoconservation, National Research Institute of Animal Production, Balice 32-083, Poland. Electronic address:
The nervous system's regenerative potential has sparked interest in exploring novel approaches to generate Schwann cell-like cells (SC-LCs) from chicken blastoderm (B)-derived embryonic stem cells (B-ESCs). This study investigates the hypothesis that specific growth factors, when used during ex-ovo culture, can induce the differentiation of chicken B-ESCs into cells resembling Schwann cells (SCs). Blastodermal cells (BCs) were isolated from in vivo-fertilized eggs at stage X followed by 14-d proliferative culture (PRC) of B-ESCs and subsequent 14-d glial/neurolemmogenic differentiation culture (DFC).
View Article and Find Full Text PDFZO-1 boosts the in vitro self-renewal of pre-haematopoietic stem cells from OCT4-reprogrammed human hair follicle mesenchymal stem cells through cytoskeleton remodeling.
Stem Cell Res Ther
December 2024
Department of Pathology, Qingdao Municipal Hospital Group, 1 Jiaozhou Road, Qingdao, 266011, Shandong, China.
Background: The challenge of expanding haematopoietic stem/progenitor cells (HSPCs) in vitro has limited their clinical application. Human hair follicle mesenchymal stem cells (hHFMSCs) can be reprogrammed to generate intermediate stem cells by transducing OCT4 (hHFMSCs) and pre-inducing with FLT3LG/SCF, and differentiated into erythrocytes. These intermediate cells exhibit gene expression patterns similar to pre-HSCs, making them promising for artificial haematopoiesis.
View Article and Find Full Text PDFPartial Reprogramming Exerts a Rejuvenating Effect on Human Mesenchymal Stem Cells That Underwent Replicative Senescence in Culture.
Int J Mol Sci
November 2024
Department of Intracellular Signaling and Transport, Institute of Cytology, Russian Academy of Sciences, Tikhoretskii pr. 4, St. Petersburg 194064, Russia.
Mesenchymal stem/stromal cells (MSCs) are becoming increasingly important for biomedical applications, such as cell therapy, disease modeling, and drug screening. At the same time, long-term cultivation, which is necessary to prepare a sufficient amount of cellular material for therapeutic and research purposes, is accompanied by the development of replicative senescence. Partial reprogramming emerged as a novel method that shows promising results in the rejuvenation of cells in vitro and in vivo; however, it has not yet been applied for human MSCs that have undergone replicative senescence in culture.
View Article and Find Full Text PDFUsing magnetic resonance relaxometry to evaluate the safety and quality of induced pluripotent stem cell-derived spinal cord progenitor cells.
Stem Cell Res Ther
December 2024
School of Chemistry, Chemical Engineering and Biotechnology, Nanyang Technological University, Singapore, Singapore.
Background: The emergence of induced pluripotent stem cells (iPSCs) offers a promising approach for replacing damaged neurons and glial cells, particularly in spinal cord injuries (SCI). Despite its merits, iPSC differentiation into spinal cord progenitor cells (SCPCs) is variable, necessitating reliable assessment of differentiation and validation of cell quality and safety. Phenotyping is often performed via label-based methods including immunofluorescent staining or flow cytometry analysis.
View Article and Find Full Text PDFRhaponticin suppresses the stemness phenotype of gastric cancer stem-like cells CD133+/CD166 + by inhibiting programmed death-ligand 1.
BMC Gastroenterol
November 2024
Department of Gastroenterology, Shaanxi Provincial People's Hospital, No. 256 Friendship West Road, Beilin District, Xi'an, Shaanxi, 710068, China.
Article Synopsis
- Gastric cancer stem cells (GCSCs) are crucial for tumor growth and recurrence, making them important targets for treatments; Rhaponticin (RA) is a new anticancer drug that may affect GCSCs' properties.
- In experiments, isolated GCSCs showed stem-like traits and were sensitive to RA, which decreased their viability and stemness markers without harming normal cells; RA showed a pronounced effect in two specific gastric cancer cell lines.
- Additionally, RA appears to target the PD-L1 gene, and studies in a mouse model demonstrated that RA significantly reduced tumor size compared to untreated GCSCs, suggesting potential therapeutic benefits for gastric cancer patients.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!