We observed a reduction of lignin content linked to the expression of fungal laccase in rice plants. The lignin content of L-4, which showed the highest LAC activity among transgenic lines produced, was lower than that of the control line. However, this change was not reflected to the saccharification efficiency.
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http://dx.doi.org/10.1016/j.jbiosc.2013.05.007 | DOI Listing |
Chem Biodivers
January 2025
Chuxiong Normal University, Academy of Science and Technology, Chuxiong Normal University, Chuxiong, 675000,China, No. 456 Luchengnan Road, chuxiong, Academy of Science and Technology, 651000, chuxiong, CHINA.
Gray mold disease is caused by B. cinerea, which could severely reduce the production yield and quality of tomatoes. To explore more potential fungicides with new scaffolds for controlling the gray mold disease, ten aldehydes-thiourea derivatives were designed, synthesized and assayed for inhibitory activity against three plant pathogenic fungi.
View Article and Find Full Text PDFJ Microbiol Methods
January 2025
Department of Biosystems and Technology, Swedish University of Agricultural Sciences, Växtskyddsvägen 3, SE-234 56 Alnarp, Sweden. Electronic address:
In recent years, oxidoreductase enzymes such as laccases have received considerable attention for their ability to degrade and eliminate organic micropollutants from contaminated water in a process known as enzyme-based wastewater treatment. Thus, methods to produce high laccase activity in water are a point of focus, with white-rot fungi being highlighted as a tool in this context. This study, therefore, explored the applied approach of direct addition of mushroom spawn of the white-rot fungi Pleurotus ostreatus into water and its potential for laccase production under different conditions.
View Article and Find Full Text PDF<b>Background and Objective:</b> Laccase as a ligninolytic enzyme has been known for its green-catalysis mechanism, which has the potential to be applied to food industries. Lignocellulose found in agro-industrial waste is promising for laccase production as a substrate, that could be encountered in pineapple (<i>Ananas comosus</i>) and Arabica coffee (<i>Coffea arabica</i>) industrial residue. To boost enzyme activity, laccase characterization was performed using <i>Ganoderma lucidum</i> under solid-state fermentation.
View Article and Find Full Text PDFBiotechnol Bioeng
January 2025
Department of Microbiology, Sanya Institute of Nanjing Agricultural University, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, China.
The three-phase structure of solid-state fermentation (SSF) directly affects substrate degradation and fermentation efficiency. However, the mechanism of three-phase regulation on lignocellulose utilization and microbial metabolism is still unclear. Based on comparative transcriptome analysis, a lignocellulose degrading enzyme, manganese peroxidase (GlMnP), which was significantly affected by water stress meanwhile related to triphase utilization, was screened to reveal the mechanism using Ganoderma lucidum as the reference strain.
View Article and Find Full Text PDFFood Sci Nutr
January 2025
Modern-day consumers are interested in highly nutritious and safe foods with corresponding organoleptic qualities. Such foods are increasingly subjected to various processing techniques which include the use of enzymes. These enzymes like amylases, lipases, proteases, xylanases, laccases, pullulanase, chitinases, pectinases, esterases, isomerases, and dehydrogenases could be derived from extremophilic organisms such as thermophiles, psychrophiles, acidophiles, alkaliphiles, and halophiles.
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