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Improved coverage and accuracy with strand-conserving sequence enrichment. | LitMetric

Improved coverage and accuracy with strand-conserving sequence enrichment.

Genome Med

Dr. John T MacDonald Department of Human Genetics and Hussman Institute for Human Genomics, Miller School of Medicine, University of Miami, FL 33136, USA.

Published: May 2014

Unlabelled: Targeted next-generation sequencing is becoming a common tool in the molecular diagnostic laboratory. However, currently available methods to enrich for regions of interest in the DNA sequence suffer from drawbacks such as high cost, complex protocols, lack of clinical-level accuracy and uneven target coverage. A target-enrichment approach using complementary long padlock probes described in a recent article significantly improves on previous methods in most of these areas.

See Related Research: http://genomemedicine.com/content/5/5/50.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3706846PMC
http://dx.doi.org/10.1186/gm450DOI Listing

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