Context: Local health departments (LHDs) were responsible for administering vaccine to the public during the 2009 H1N1 campaign but had relatively little guidance or experience to inform such a large campaign with limited vaccine supply. They used various processes to deliver vaccines to the public. Learning from the LHDs that were successful in their public vaccination campaigns can help inform future vaccination efforts.
Objective: To learn from H1N1 campaign in order to improve responses to similar events in the future. We used a positive deviance approach to identify positive outlier LHDs during the 2009 H1N1 vaccination campaigns. We then used realist evaluation to learn about the combinations of context and mechanisms that led select LHDs to perform well.
Design: This project was a retrospective qualitative study. We used process mapping to define the steps involved in implementing public vaccination clinics. We identified positive outliers in H1N1 public vaccination efforts and conducted in-depth interviews with LHD staff to learn about the context and mechanisms that led to successful public vaccination clinics.
Participants: This study focused on positive outliers in LHD vaccination efforts during the 2009 H1N1 campaign that implemented public vaccination clinics.
Main Outcome Measure(s): Study outcomes focused on the combinations of context and mechanisms that led to successful outcomes in high-performing LHDs. Specific context + mechanism = outcomes stories are reported.
Results: We found that successful LHDs defined priority groups, communicated with the public, maintained adequate staffing, established community partnerships, and maintained flexibility. We describe the specific contexts that triggered key mechanisms, which, when combined, led to successful outcomes.
Conclusions: The positive deviance and realist evaluation approach allowed us to understand how LHD context triggered specific mechanisms, which led to successful public clinics. The experience of successful LHDs can assist similar LHDs in implementing successful public vaccination clinics in the future.
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http://dx.doi.org/10.1097/PHH.0b013e318269e434 | DOI Listing |
Sci Transl Med
January 2025
Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA.
At this stage in the COVID-19 pandemic, most infections are "breakthrough" infections that occur in individuals with prior severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) exposure. To refine long-term vaccine strategies against emerging variants, we examined both innate and adaptive immunity in breakthrough infections. We performed single-cell transcriptomic, proteomic, and functional profiling of primary and breakthrough infections to compare immune responses from unvaccinated and vaccinated individuals during the SARS-CoV-2 Delta wave.
View Article and Find Full Text PDFSci Adv
January 2025
Department of Microbiology and Immunology, Cornell University College of Veterinary Medicine, Ithaca, NY 14853, USA.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and influenza viruses lead to severe respiratory illnesses and death in humans, exacerbated in individuals with underlying health conditions, remaining substantial global public health concerns. Here, we developed a bivalent replication-incompetent single-cycle pseudotyped vesicular stomatitis virus vaccine that incorporates both a prefusion-stabilized SARS-CoV-2 spike protein lacking a furin cleavage site and a full-length influenza A virus neuraminidase protein. Vaccination of K18-hACE2 or C57BL/6J mouse models generated durable levels of neutralizing antibodies, T cell responses, and protection from morbidity and mortality upon challenge with either virus.
View Article and Find Full Text PDFPLoS One
January 2025
Instituto René Rachou, Fiocruz Minas, Fundação Oswaldo Cruz (Fiocruz), Belo Horizonte, Minas Gerais, Brazil.
Background: To develop an effective vaccine against Plasmodium vivax, the most widely dispersed human malaria parasite, it is critical to understand how coinfections with other pathogens could impact malaria-specific immune response. A recent conceptual study proposed that Epstein-Barr virus (EBV), a highly prevalent human herpesvirus that establishes lifelong persistent infection, may influence P. vivax antibody responses.
View Article and Find Full Text PDFPLOS Glob Public Health
January 2025
[This corrects the article DOI: 10.1371/journal.pgph.
View Article and Find Full Text PDFArch Microbiol
January 2025
Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor, 43400, Malaysia.
Bacteriophages produce endolysins at the end of the lytic cycle, which are crucial for lysing the host cells and releasing virion progeny. This lytic feature allows endolysins to act as effective antimicrobial alternatives when applied exogenously. Staphylococcal endolysins typically possess a modular structure with one or two enzymatically active N-terminal domains (EADs) and a C-terminal cell wall binding domain (CBD).
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