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Using DIMet for Differential Analysis of Labeled Metabolomics Data: A Step-by-step Guide Showcasing the Glioblastoma Metabolism.
Bio Protoc
January 2025
University of Bordeaux, CNRS, IBGC UMR 5095, Bordeaux, France.
Stable-isotope resolved metabolomics (SIRM) is a powerful approach for characterizing metabolic states in cells and organisms. By incorporating isotopes, such as C, into substrates, researchers can trace reaction rates across specific metabolic pathways. Integrating metabolomics data with gene expression profiles further enriches the analysis, as we demonstrated in our prior study on glioblastoma metabolic symbiosis.
View Article and Find Full Text PDFThe James Webb Space Telescope has discovered a surprising population of bright galaxies in the very early Universe (≲500 Myr after the Big Bang) that is hard to explain with conventional galaxy-formation models and whose physical properties are not fully understood. Insight into their internal physics is best captured through nebular lines, but at these early epochs, the brightest of these spectral features are redshifted into the mid-infrared and remain elusive. Using the mid-infrared instrument onboard the James Webb Space Telescope, here we present a detection of Hα and doubly ionized oxygen ([O iii] 4959,5007 Å) from the bright, ultra-high-redshift galaxy candidate GHZ2/GLASS-z12.
View Article and Find Full Text PDFPollution resistance of Saturn's ring particles during micrometeoroid impact.
Nat Geosci
December 2024
Université Paris Cité, Institut de Physique du Globe de Paris, CNRS, Paris, France.
Saturn's rings have been estimated to be as young as about 100 to 400 million years old according to the hypothesis that non-icy micrometeoroid bombardment acts to darken the rings over time and the Cassini observation indicated that the ring particles appear to be relatively clean. These young age estimates assume that the rings formed out of pure water ice particles with a high accretion efficiency of impacting non-icy micrometeoroid material ( ≳ 10%). Here we show, using numerical simulations of hypervelocity micrometeoroid impacts on a ring particle, that non-icy material may not be as readily accreted as previously thought.
View Article and Find Full Text PDFDecontamination of DNA sequences from a Streptomyces genome for optimal genome mining.
Braz J Microbiol
January 2025
Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo (USP), São Paulo, SP, 05508-900, Brazil.
Despite meticulous precautions, contamination of genomic DNA samples is not uncommon, which can significantly compromise the analysis of microorganisms' whole-genome sequencing data, thus affecting all subsequent analyses. Thanks to advancements in software and bioinformatics techniques, it is now possible to address this issue and prevent the loss of the entire dataset obtained in a contaminated whole-genome sequencing, where the DNA of another bacterium is present. In this study, it was observed that the sequencing reads from Streptomyces sp.
View Article and Find Full Text PDFDesign and implementation of optics for the experiment for cryogenic large-aperture intensity mapping (EXCLAIM).
Rev Sci Instrum
January 2025
NASA Goddard Space Flight Center, Greenbelt, Maryland 20771, USA.
This work describes the design and implementation of optics for EXCLAIM, the EXperiment for Cryogenic Large-Aperture Intensity Mapping. EXCLAIM is a balloon-borne telescope that will measure integrated line emission from carbon monoxide at redshifts z < 1 and ionized carbon ([CII]) at redshifts z = 2.5 - 3.
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