We report the development of molecularly imprinted polyacrylamide nanoparticles that bind to and neutralize the activity of cytotoxins present in the venom of the Mozambique Spitting Cobra (Naja mossambica mossambica). The binding activity of these nanoparticles is avid and specific. These findings hold promise for the development of a synthetic antivenom.
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http://dx.doi.org/10.1039/c3cc42394h | DOI Listing |
PLoS Negl Trop Dis
April 2024
Department of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszow University of Technology, Rzeszow, Poland.
Background: Intraspecific variations in snake venom composition have been extensively documented, contributing to the diverse clinical effects observed in envenomed patients. Understanding these variations is essential for developing effective snakebite management strategies and targeted antivenom therapies. We aimed to comprehensively investigate venoms from three distinct populations of N.
View Article and Find Full Text PDFRes Pract Thromb Haemost
January 2024
Department of Physiology, School of Medicine, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.
Background: Venomous snake bites have been listed as a neglected tropical disease by the World Health Organization. The Mozambique spitting cobra ( is found in Sub-Saharan African countries, and its venom has been identified to predominantly result in cytotoxic effects. However, there is limited evidence on the possible hemotoxic effects of this venom on human blood.
View Article and Find Full Text PDFInt J Mol Sci
November 2023
Centre for Analytical Sciences Amsterdam (CASA), 1012 WX Amsterdam, The Netherlands.
Snakebite is considered a concerning issue and a neglected tropical disease. Three-finger toxins (3FTxs) in snake venoms primarily cause neurotoxic effects since they have high affinity for nicotinic acetylcholine receptors (nAChRs). Their small molecular size makes 3FTxs weakly immunogenic and therefore not appropriately targeted by current antivenoms.
View Article and Find Full Text PDFJ Proteome Res
June 2023
Amsterdam Institute of Molecular and Life Sciences, Division of BioAnalytical Chemistry, Department of Chemistry and Pharmaceutical Sciences, Faculty of Science, Vrije Universiteit Amsterdam, De Boelelaan 1085, Amsterdam 1081HV, The Netherlands.
In this study, we present high-throughput (HT) venomics, a novel analytical strategy capable of performing a full proteomic analysis of a snake venom within 3 days. This methodology comprises a combination of RP-HPLC-nanofractionation analytics, mass spectrometry analysis, automated in-solution tryptic digestion, and high-throughput proteomics. In-house written scripts were developed to process all the obtained proteomics data by first compiling all Mascot search results for a single venom into a single Excel sheet.
View Article and Find Full Text PDFMicroorganisms
September 2022
Water Research Group, Unit for Environmental Sciences and Management, North-West University, Potchefstroom 2531, South Africa.
Reptiles are hosts for various tick species and tick-associated organisms, many of which are zoonotic. However, little is known about the presence and diversity of tick-borne bacteria infecting reptiles and their ticks in South Africa. ticks ( = 253) collected from reptiles were screened for the presence of , , and species by amplification, sequencing and phylogenetic analysis of the 16S rRNA, 23S rRNA, , , and genes, respectively.
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