A yeast strain was isolated from olive brines in a fermented black olive and olive oil manufacturing plant in the town of Nyons (France). On the basis of domains 1 and 2 (D1/D2) large subunit (LSU) rRNA gene and internal transcribed spacer (ITS) region sequence analyses, the strain CLIB 1303(T) was found to be closely related, but clearly distinct, from the three existing species of the genus Citeromyces: Citeromyces matritensis, Citeromyces siamensis and Citeromyces haiwaiiensis. Strain CLIB 1303(T) exhibited 6 bp, 7 bp and 12 bp divergences in the D1/D2 LSU rRNA gene with C. siamensis, C. matritensis and C. hawaiiensis, respectively. ITS region divergence amounted to more than 8 %, 4 % and 4.5 % with C. siamensis, C. matritensis and C. hawaiiensis, respectively, in addition to several indels. Like C. matritensis and C. siamensis strains, strain CLIB 1303(T) was shown to be halotolerant and osmotolerant. Phenotypically, strain CLIB 1303(T) can be distinguished from other species of the genus Citeromyces by its inability to assimilate trehalose. The strain CLIB 1303(T) (= CBS 12700(T)) was assigned to a novel species, Citeromyces nyonsensis sp. nov.
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http://dx.doi.org/10.1099/ijs.0.049296-0 | DOI Listing |
Microbiol Resour Announc
December 2022
SPO, Univ Montpellier, INRAE, Institut Agro, Montpellier, France.
Here, we report the draft genome sequence and annotation of the yeast Candida railenensis strain CLIB 1423. The assembly consists of 57 nuclear scaffolds and 1 complete mitochondrial chromosome, for a total of 13.8 Mb (, 0.
View Article and Find Full Text PDFBiotechnol Bioeng
January 2023
Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Jülich, Germany.
Extracellular production of target proteins simplifies downstream processing due to obsolete cell disruption. However, optimal combinations of a heterologous protein, suitable signal peptide, and secretion host can currently not be predicted, resulting in large strain libraries that need to be tested. On the experimental side, this challenge can be tackled by miniaturization, parallelization, and automation, which provide high-throughput screening data.
View Article and Find Full Text PDFMetabolites
May 2022
University Grenoble Alpes, CNRS, UMR5525, VetAgro Sup, Grenoble INP, TIMC, 38000 Grenoble, France.
Coenzyme Q (CoQ) is a lipid-soluble compound with important physiological functions and is sought after in the food and cosmetic industries owing to its antioxidant properties. In our previous proof of concept, we engineered for CoQ biosynthesis the industrially relevant , which does not naturally synthesize any CoQ. Here, liquid chromatography-mass spectrometry (LC-MS) analysis identified two metabolic bottlenecks in the CoQ production, i.
View Article and Find Full Text PDFGenome Biol Evol
February 2022
Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Jouy-en-Josas, France.
Recent studies have suggested that species of the Kazachstania genus may be interesting models of yeast domestication. Among these, Kazachstania barnettii has been isolated from various microbially transformed foodstuffs such as sourdough bread and kefir. In the present work, we sequence, assemble, and annotate the complete genomes of two K.
View Article and Find Full Text PDFMicrob Cell Fact
September 2021
Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Jülich, Germany.
Background: The split GFP assay is a well-known technology for activity-independent screening of target proteins. A superfolder GFP is split into two non-fluorescent parts, GFP11 which is fused to the target protein and GFP1-10. In the presence of both, GFP1-10 and the GFP11-tag are self-assembled and a functional chromophore is formed.
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