AI Article Synopsis
- - The study focuses on detecting measles-specific IgG antibodies using electrochemical impedimetric immunosensors, achieving optimal electrode conditions after 40 minutes.
- - Surface roughness, measured by AFM, increased with each step of immobilization or antigen-antibody reaction, confirming successful surface modifications and interactions.
- - Human serum and new-born calf serum were tested to simulate real-world samples, showing a correlation between antibody concentrations and charge-transfer resistance, highlighting the potential of impedance methods for label-free detection of measles antibodies.
Article Abstract
The detection of measles-specific primary antibodies (IgG) using electrochemical impedimetric immunosensors is reported. The optimum conditions for electrode saturation were reached after 40 min for 1 μg ml(-1) antibody concentrations. Surface roughness using AFM increased with each immobilization or antigen-antibody reaction step clearly confirming the surface modification and recognition between antigen and antibody. The human serum (HS) and new-born calf serum (NCS) spiked with antigen-specific antibody were studied to mimic the real sample analysis. The HS and NCS sera containing antibodies due to measles exhibited correlation between the increasing antibody serum concentrations and the charge-transfer resistance (electrochemically measured). This work clearly showed the potential use of impedance as the preferred electrochemical method for detecting measles-antibodies in label-free manner.
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| http://dx.doi.org/10.1016/j.bios.2013.04.028 | DOI Listing |
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