Purpose: To explore the characters of attachment, proliferation and osteogenic differentiation of stem cells from goat deciduous teeth (SGDs) cultured on akermanite ceramic.
Methods: SGDs were collected with enzyme digestion, and cultured. Under culture condition in growth medium, actin filament labeling and CCK-8 analysis were used to reveal attachment and proliferation. Expression level of alkaline phosphatase (ALP) and osteogenic differentiation genes (ALP, COL I, OPN and OCN) were examined by pNPP method and real-time PCR. The data was analyzed using SPSS 13.0 software package.
Results: Compared with β-TCP, SGDs seeded on akermanite ceramic not only spread out better, but also grew faster since day 4. In osteogenic medium, cells of akermanite group had stronger ALP activity at day 4 and 7. The gene expression of akermanite was more than the β-TCP group. The gene OCN expression level increased significantly at day 7 (P<0.01).
Conclusions: SGDs can attach and grow well on akermanite ceramic. Compared with β-TCP, akermanite ceramic shows an advantage in osteogenic differentiation of SGDs.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
High-intensity focused ultrasound: an innovative approach for micro-manipulation of demineralized dentine.
J Transl Med
January 2025
Dental School, The University of Western Australia, 17 Monash Avenue, Nedlands, WA, 6009, Australia.
Background: Treatment of deep carious lesions poses significant challenges in dentistry, as complete lesion removal risks compromising pulp vitality, while selective removal often reduces the longevity of restorations. Herein, we propose a minimally invasive approach using High-Intensity Focused Ultrasound (HIFU) for microscale removal of carious dentine. Concurrently, HIFU's antimicrobial effects against associated cariogenic biofilms and the corresponding thermal and biological impacts on surrounding tissues were investigated.
View Article and Find Full Text PDFPancreatic organogenesis mapped through space and time.
Exp Mol Med
January 2025
Program in Developmental Biology, Baylor College of Medicine, Houston, TX, USA.
The spatial organization of cells within a tissue is dictated throughout dynamic developmental processes. We sought to understand whether cells geometrically coordinate with one another throughout development to achieve their organization. The pancreas is a complex cellular organ with a particular spatial organization.
View Article and Find Full Text PDFStargardt disease is a currently untreatable, inherited neurodegenerative disease that leads to macular degeneration and blindness due to loss-of-function mutations in the ABCA4 gene. We have designed a dual adeno-associated viral vector encoding a split-intein adenine base editor to correct the most common mutation in ABCA4 (c.5882G>A, p.
View Article and Find Full Text PDFβ-Glucan reprograms neutrophils to promote disease tolerance against influenza A virus.
Nat Immunol
January 2025
Department of Medicine, Department of Pathology, Department of Microbiology & Immunology, McGill University Health Centre, McGill International TB Centre, Meakins Christie Laboratories, McGill University, Montréal, Québec, Canada.
Disease tolerance is an evolutionarily conserved host defense strategy that preserves tissue integrity and physiology without affecting pathogen load. Unlike host resistance, the mechanisms underlying disease tolerance remain poorly understood. In the present study, we investigated whether an adjuvant (β-glucan) can reprogram innate immunity to provide protection against influenza A virus (IAV) infection.
View Article and Find Full Text PDFMolecular and cellular dynamics of the developing human neocortex.
Nature
January 2025
The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, San Francisco, CA, USA.
The development of the human neocortex is highly dynamic, involving complex cellular trajectories controlled by gene regulation. Here we collected paired single-nucleus chromatin accessibility and transcriptome data from 38 human neocortical samples encompassing both the prefrontal cortex and the primary visual cortex. These samples span five main developmental stages, ranging from the first trimester to adolescence.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!