Purpose: To investigate the mechanism and approach related to persisters emerging in Candida albicans biofilm by studying its dynamic characteristics.
Methods: The Candida albicans biofilms model in vitro were formed by individual yeast cells and hyphal compartments respectively. The numbers of progenitive fungal cells and persisters at different stages were counted and analyzed with SPSS11.5 software package. The biofilm morphological changes were observed under confocal scanning laser microscopy.
Results: Different stages of biofilms which were formed by individual yeast cells and hyphal compartments had no significant difference both on the number of progenitive fungal cells and persisters. The number of progenitive fungal cells showed S-shaped growth curve until 12 hours. The persisters emerged within 0.5 hour and its number reached a steady state after 2 hours while the biofilm was in the period of microcolony formation under microscopy.
Conclusions: The persisters emerging in Candida albicans biofilm are closely connected with the attachment to a surface(within 2 hours of the biofilm formation) and have little association with the maturity of the biofilm and its dimorphism.
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